Using RAPDs to study phylogenetic relationships in Rosa

被引：134

作者：

Millan, T ^{[1
]}

Osuna, F ^{[1
]}

Cobos, S ^{[1
]}

Torres, AM ^{[1
]}

Cubero, JI ^{[1
]}

机构：

[1] CIDA,DEPT MEJORA & AGRON,E-14080 CORDOBA,SPAIN

来源：

THEORETICAL AND APPLIED GENETICS | 1996年 / 92卷 / 02期

关键词：

Rosa sp; RAPDs markers; polymerase chain reaction; phylogenetic relationships;

D O I：

10.1007/BF00223385

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Nineteen species of rose (Rosa sp.) were analysed using Random Amplified Polymorphic DNA markers (RAPD). Each 10-base-long arbitrary primer produced a specific DNA banding pattern that grouped plants belonging to the same species and botanical sections as predicted from their genetic background. One hundred and seventy-five amplification products were examined by cluster analysis to assess the genetic relationships among species and their genetic distances. All of the accessions belonging to 1 species grouped together before branching to other species. Dendrograms constructed for intra- and inter-specific studies showed a good correlation with previous classifications by different authors based on morphological and cariological studies. Our results show that the RAPD technique is a sensitive and precise tool for genomic analysis in rose, being useful in assigning unclassified accessions to specific taxonomic groups or else allowing accessions classified by traditional criteria to be re-classified.

引用

页码：273 / 277

页数：5

共 25 条

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