Adhesion molecules expressed by activated endothelial cells play key role in regulating leukocyte trafficking to sites of inflammation. The present study attempted to explore whether the polyphenolic flavonoid quercetin influence leukocyte endothelial attraction and the involvement of TLR-NF-kappa B signaling pathway in the expression of adhesion molecules involved in the early development of atherosclerosis. Quercetin at 25 mu M concentration significantly reduced the HUVEC expression of VCAM-1 and ICAM-1 evidently enhanced by oxLDL. In addition, quercetin significantly downregulated the mRNA expression of MCP-1 and alleviated the nuclear translocation of NF-kappa B p65 subunit in oxLDL induced HUVECs. Western blot and PCR analyses revealed that quercetin significantly attenuated the expression of both protein and mRNA expression of TLR2 and TLR4. Quercetin supplementation significantly decreased the inflammatory mediators like COX, 5-LOX, MPO, NOS, CRP and the mRNA expression of the cytokine; IL-6 in hypercholesterolemic diet (HCD) fed atherosclerotic rats. The results demonstrate that quercetin is effective to regulate the atherosclerotic inflammatory process by inhibiting oxLDL induced endothelial leukocyte adhesion by attenuating the TLR-NF-kappa B signaling pathway in endothelial cells and decrease the inflammatory process induced by HCD in rats. Therefore, quercetin acts as anti-inflammatory and anti-atherogenic agent, which may have implications for strategies attenuating endothelial dysfunction-related atherosclerosis. (C) 2016 Elsevier Inc. All rights reserved.
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Sree Chitra Tirunal Inst Med Sci & Technol, Div Cellular & Mol Cardiol, Trivandrum, Kerala, IndiaSree Chitra Tirunal Inst Med Sci & Technol, Div Cellular & Mol Cardiol, Trivandrum, Kerala, India
Panicker, Sumith Retnamma
Sreenivas, Prethish
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Natl Ctr Biol Sci, Bangalore, Karnataka, IndiaSree Chitra Tirunal Inst Med Sci & Technol, Div Cellular & Mol Cardiol, Trivandrum, Kerala, India
Sreenivas, Prethish
Babu, Mani Sankar
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机构:Sree Chitra Tirunal Inst Med Sci & Technol, Div Cellular & Mol Cardiol, Trivandrum, Kerala, India
Babu, Mani Sankar
Karunagaran, Devarajan
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Indian Inst Technol, Dept Biotechnol, Madras 600036, Tamil Nadu, IndiaSree Chitra Tirunal Inst Med Sci & Technol, Div Cellular & Mol Cardiol, Trivandrum, Kerala, India
Karunagaran, Devarajan
Kartha, Chandrasekharan Cheranellore
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机构:Sree Chitra Tirunal Inst Med Sci & Technol, Div Cellular & Mol Cardiol, Trivandrum, Kerala, India
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Karolinska Inst, Div Physiol Chem 2, Dept Med Biochem & Biophys, S-17177 Stockholm, SwedenKarolinska Inst, Div Physiol Chem 2, Dept Med Biochem & Biophys, S-17177 Stockholm, Sweden
Radmark, Olof
Samuelsson, Bengt
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Karolinska Inst, Div Physiol Chem 2, Dept Med Biochem & Biophys, S-17177 Stockholm, SwedenKarolinska Inst, Div Physiol Chem 2, Dept Med Biochem & Biophys, S-17177 Stockholm, Sweden
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Sree Chitra Tirunal Inst Med Sci & Technol, Div Cellular & Mol Cardiol, Trivandrum, Kerala, IndiaSree Chitra Tirunal Inst Med Sci & Technol, Div Cellular & Mol Cardiol, Trivandrum, Kerala, India
Panicker, Sumith Retnamma
Sreenivas, Prethish
论文数: 0引用数: 0
h-index: 0
机构:
Natl Ctr Biol Sci, Bangalore, Karnataka, IndiaSree Chitra Tirunal Inst Med Sci & Technol, Div Cellular & Mol Cardiol, Trivandrum, Kerala, India
Sreenivas, Prethish
Babu, Mani Sankar
论文数: 0引用数: 0
h-index: 0
机构:Sree Chitra Tirunal Inst Med Sci & Technol, Div Cellular & Mol Cardiol, Trivandrum, Kerala, India
Babu, Mani Sankar
Karunagaran, Devarajan
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机构:
Indian Inst Technol, Dept Biotechnol, Madras 600036, Tamil Nadu, IndiaSree Chitra Tirunal Inst Med Sci & Technol, Div Cellular & Mol Cardiol, Trivandrum, Kerala, India
Karunagaran, Devarajan
Kartha, Chandrasekharan Cheranellore
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h-index: 0
机构:Sree Chitra Tirunal Inst Med Sci & Technol, Div Cellular & Mol Cardiol, Trivandrum, Kerala, India
机构:
Karolinska Inst, Div Physiol Chem 2, Dept Med Biochem & Biophys, S-17177 Stockholm, SwedenKarolinska Inst, Div Physiol Chem 2, Dept Med Biochem & Biophys, S-17177 Stockholm, Sweden
Radmark, Olof
Samuelsson, Bengt
论文数: 0引用数: 0
h-index: 0
机构:
Karolinska Inst, Div Physiol Chem 2, Dept Med Biochem & Biophys, S-17177 Stockholm, SwedenKarolinska Inst, Div Physiol Chem 2, Dept Med Biochem & Biophys, S-17177 Stockholm, Sweden