Label-free aptasensor for thrombin determination based on the nanostructured phenazine mediator

被引：15
作者：
Evtugyn, Gennady A. [1 ]
Kostyleva, Veronika B. [1 ,2 ]
Porfireva, Anna V. [1 ]
Savelieva, Maria A. [1 ]
Evtugyn, Vladimir G. [3 ]
Sitdikov, Rusal R. [4 ]
Stoikov, Ivan I. [4 ]
Antipin, Igor S. [4 ]
Hianik, Tibor [2 ]
机构：
[1] Kazan Fed Univ, Dept Analyt Chem, Kazan 420008, Russia
[2] Comenius Univ, Dept Nucl Phys & Biophys, Bratislava 84248, Slovakia
[3] Kazan Fed Univ, Fac Biol, Electron Microscopy Lab, Kazan 420008, Russia
[4] Kazan Fed Univ, Dept Organ Chem, Kazan 420008, Russia
来源：
TALANTA | 2012年 / 102卷
基金：
俄罗斯基础研究基金会;
关键词：
Aptasensor; Thrombin; DNA aptamer; Thiacalixarene; Neutral Red; AMPLIFIED ELECTROCHEMICAL APTASENSOR; CARBON NANOTUBES; GOLD NANOPARTICLES; MOLECULAR BEACON; HUMAN BLOOD; APTAMER; PLATFORM; REAGENTLESS; BIOSENSOR; SWITCH;
D O I：
10.1016/j.talanta.2012.07.007
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
New aptasensors based on DNA aptamer and polycarboxylated thiacalix[4]arenes in cone, 1,3-alternate and partial cone configurations bearing Neutral Red (NR) at substituents at the lower rim have been developed and applied for thrombin detection. The assembly of the biorecognition layer was optimized by AFM and EIS study to reach the maximal coverage and regular composition of the surface layer. The interaction of the NR groups with thrombin suppressed the electron hopping between oxidized and reduced mediator groups. This regularly decreased the NR peak current and increased the resistance of the charge transfer. The aptasensor makes it possible to detect from 1 nM to 1 mu M of thrombin with the detection limit of 0.05-0.5 nM. No effect of the 1000 excess of bovine serum albumin on the signal was observed. The influence of thiacalix[4]arene configuration on the sensitivity of aptasensor signal toward thrombin is discussed. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码：156 / 163
页数：8
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