Dietary L-arginine supplementation alleviates liver injury caused by Escherichia coli LPS in weaned pigs

被引:65
作者
Li, Quan [1 ]
Liu, Yulan [1 ,2 ]
Che, Zhengquan [1 ]
Zhu, Huiling [1 ]
Meng, Guoquan [1 ]
Hou, Yongqing [1 ]
Ding, Bingying
Yin, Yulong [1 ,2 ]
Chen, Feng [1 ]
机构
[1] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China
[2] Chinese Acad Sci, Inst Subtrop Agr, Changsha, Hunan, Peoples R China
基金
中国国家自然科学基金;
关键词
Arginine; weaned pigs; lipopolysaccharide; Toll-like receptor 4; nuclear factor-kappa B; liver injury; NITRIC-OXIDE; ISCHEMIA-REPERFUSION; AMINO-ACIDS; LIPOPOLYSACCHARIDE; NUTRITION; STRESS; METABOLISM; MODULATION; ACTIVATION; EXPRESSION;
D O I
10.1177/1753425912441955
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
This study was conducted to evaluate whether dietary supplementation with L-arginine (Arg) could attenuate Escherichia coli LPS-induced liver injury through the TLR4 signaling pathway in weaned pigs. Eighteen weaned pigs were allotted to three treatments: non-challenged control, LPS challenged control and LPS + 0.5% Arg. On d 18, pigs were injected with LPS at 100 mu g/kg of body weight (BW) or sterile saline. Blood samples were obtained at 4 h post-injection. Pigs were then sacrificed for the collection of liver samples. Arg supplementation (0.5%) alleviated liver morphological impairment, including hepatocyte caryolysis, karyopycnosis and fibroblast proliferation induced by LPS challenge; it mitigated the increase of serum aspartate aminotransferase and alkaline phosphatase activities induced by LPS (P < 0.05); it prevented the increase of hepatic TNF-alpha, malondialdehyde contents and mast cell number induced by LPS administration (P < 0.05); and it attenuated the elevation of hepatic NF-kappa B and TLR4-positive cell percentages (P < 0.05). These results indicate that Arg supplementation has beneficial effects in attenuating hepatic morphological and functional injury induced by LPS challenge in piglets. Additionally, it is possible that the protective effects of Arg on the liver are associated with a decreased release of liver pro-inflammatory cytokines and free radicals through inhibiting TLR4 signaling.
引用
收藏
页码:804 / 814
页数:11
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