Immunogold Detection of L-glutamate and D-serine in Small Synaptic-Like Microvesicles in Adult Hippocampal Astrocytes

被引：79

作者：

Bergersen, L. H. ^{[1
]}

Morland, C. ^{[1
]}

Ormel, L. ^{[1
]}

Rinholm, J. E. ^{[1
]}

Larsson, M. ^{[1
]}

Wold, J. F. H. ^{[1
]}

Roe, A. T. ^{[1
]}

Stranna, A. ^{[1
]}

Santello, M. ^{[2
]}

Bouvier, D. ^{[2
]}

Ottersen, O. P. ^{[1
]}

Volterra, A. ^{[2
]}

Gundersen, V. ^{[1
,3
]}

机构：

[1] Univ Oslo, Dept Anat, Ctr Mol Biol & Neurosci, Inst Basic Med Sci, N-0317 Oslo, Norway

[2] Univ Lausanne, Dept Biol Cellulaire & Morphol, CH-1005 Lausanne, Switzerland

[3] Natl Hosp Norway, Oslo Univ Hosp, Dept Neurol, N-0424 Oslo, Norway

来源：

CEREBRAL CORTEX | 2012年 / 22卷 / 07期

基金：

瑞士国家科学基金会;

关键词：

gliotransmitter; gold particles; rat; ultrastructure; LONG-TERM POTENTIATION; VESICULAR COMPARTMENT; NEURONAL-ACTIVITY; CALCIUM SIGNALS; AMINO-ACIDS; RAT-BRAIN; IN-SITU; EXOCYTOSIS; RELEASE; QUANTIFICATION;

D O I：

10.1093/cercor/bhr254

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Glutamate and the N-methyl-D-aspartate receptor ligand D-serine are putative gliotransmitters. Here, we show by immunogold cytochemistry of the adult hippocampus that glutamate and D-serine accumulate in synaptic-like microvesicles (SLMVs) in the perisynaptic processes of astrocytes. The estimated concentration of fixed glutamate in the astrocytic SLMVs is comparable to that in synaptic vesicles of excitatory nerve terminals (similar to 45 and similar to 55 mM, respectively), whereas the D-serine level is about 6 mM. The vesicles are organized in small spaced clusters located near the astrocytic plasma membrane. Endoplasmic reticulum is regularly found in close vicinity to SLMVs, suggesting that astrocytes contain functional nanodomains, where a local Ca2+ increase can trigger release of glutamate and/or D-serine.

引用

页码：1690 / 1697

页数：8

共 66 条

[51] SYNAPTIC MODULATION BY ASTROCYTES VIA Ca2+-DEPENDENT GLUTAMATE RELEASE [J].

Santello, M. ;

Volterra, A. .

NEUROSCIENCE, 2009, 158 (01) :253-259

[52] TNFα Controls Glutamatergic Gliotransmission in the Hippocampal Dentate Gyrus [J].

Santello, Mirko ;

Bezzi, Paola ;

Volterra, Andrea .

NEURON, 2011, 69 (05) :988-1001

[53] D-SERINE, AN ENDOGENOUS SYNAPTIC MODULATOR - LOCALIZATION TO ASTROCYTES AND GLUTAMATE-STIMULATED RELEASE [J].

SCHELL, MJ ;

MOLLIVER, ME ;

SNYDER, SH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (09) :3948-3952

[54] Tuned responses of astrocytes and their influence on hemodynamic signals in the visual cortex [J].

Schummers, James ;

Yu, Hongbo ;

Sur, Mriganka .

SCIENCE, 2008, 320 (5883) :1638-1643

[55] A genetically targeted optical sensor to monitor calcium signals in astrocyte processes [J].

Shigetomi, Eiji ;

Kracun, Sebastian ;

Sofroniew, Michael V. ;

Khakh, Baljit S. .

NATURE NEUROSCIENCE, 2010, 13 (06) :759-U143

[56]

SHUPLIAKOV O, 1992, J NEUROSCI, V12, P3789

[57] D-serine and serine racemase are present in the vertebrate retina and contribute to the physiological activation of NMDA receptors [J].

Stevens, ER ;

Esguerra, M ;

Kim, PM ;

Newman, EA ;

Snyder, SH ;

Zahs, KR ;

Miller, RF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (11) :6789-6794

[58] 1ST VISUALIZATION OF GLUTAMATE AND GABA IN NEURONS BY IMMUNOCYTOCHEMISTRY [J].

STORMMATHISEN, J ;

LEKNES, AK ;

BORE, AT ;

VAALAND, JL ;

EDMINSON, P ;

HAUG, FMS ;

OTTERSEN, OP .

NATURE, 1983, 301 (5900) :517-520

[59] Three-dimensional relationships between hippocampal synapses and astrocytes [J].

Ventura, R ;

Harris, KM .

JOURNAL OF NEUROSCIENCE, 1999, 19 (16) :6897-6906

[60] Astrocytes, from brain glue to communication elements: The revolution continues [J].

Volterra, A ;

Meldolesi, J .

NATURE REVIEWS NEUROSCIENCE, 2005, 6 (08) :626-640

← 1 2 3 4 5 6 7 →