Fructosyl amine sensing based on Prussian blue modified enzyme electrode

Low potential amperometric enzyme sensor for fructosyl-valine, a model compound of glycated hemoglobin (HbAlc), was constructed using a fructosyl-amine oxidase (FAO) and Prussian blue film as the artificial peroxidase. The Prussian blue-based FAO electrode exhibits a good linear correlation between 0.1 to 0.3 mM, similar to what was observed with the low potential enzyme electrode employing FAO, peroxidase, and ferrocene as the electron mediator. These results indicated that the Prussian blue-based FAO electrode may avoid the inherent problems of hydrogen peroxide measurement at the high potential and also have advantage in its simple construct of the system compared with the two enzyme peroxidase - FAO - ferrocene system.