Measurement of cholesterol low-density lipoprotein using three methodologies

被引:0
作者
Querales, Marvin [1 ]
Elena Cruces, Maria [1 ]
Sanchez, Cesar [1 ]
Querales, Marisel [1 ]
Rojas, Susan [1 ]
Sanchez, Lissette [1 ]
机构
[1] Univ Carabobo, Sect La Ceiba, Fac Ciencias Salud, Escuela Ciencias Biomed & Tecnol,Dept Bioquim, Valencia, Venezuela
来源
ACTA BIOQUIMICA CLINICA LATINOAMERICANA | 2012年 / 46卷 / 01期
关键词
low density lipoproteins; homogeneous enzymatic method; precipitation method; Friedewald formula; LDL-CHOLESTEROL; ASSAYS; RISK;
D O I
暂无
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Considering that there is still no standard methodology for routine determination of low density lipoprotein (LDL-c) it was decided to evaluate their analytical determination using three techniques: homogeneous enzymatic determination, polyvinyl sulphate precipitation and Friedewald formula. Ninety-eight serum samples were processed; triglycerides (TG), total cholesterol (TC), high-density lipoprotein (HDL-c) and LDL-c were determined. Mean total cholesterol was 194.46 +/- 43.54 mg/dL, HDL-C was 51.12 +/- 12.36 mg/dL and TG was 132.88 +/- 76.93 mg/dL. Although regression analysis showed a good correlation between LDL-c, the results showed a statistically significative difference in them when TG levels exceeded 200 mg/dL. It was mainly observed in the precipitation method and the Friedewald formula, the latter values being significantly lower (LDL-C by precipitation: 141.3 +/- 26.2 mg/dL, LDL-C by the Friedewald formula: 110, 1 +/- 35.4 mg/dL). Moreover, this difference affected the proportion of individuals classified according to their coronary risk. It is necessary to compare the techniques applied in this study with beta quantification to assess which has a higher level of accuracy.
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收藏
页码:31 / 37
页数:7
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