Determination of genetic changes in etiology of autism spectrum disorder in twins by whole-exome sequencing

被引：5

作者：

Hayretdag, Ceyda ^{[1
]}

Algedik, Pinar ^{[2
,4
]}

Ekmekci, Cumhur Gokhan ^{[3
]}

Gunal, Ozlem Bozdagi ^{[4
]}

Agyuz, Umut ^{[5
]}

Yildirim, Halime ^{[6
]}

Coskunpinar, Ender ^{[4
,6
]}

机构：

[1] Univ Hlth Sci, Sch Med, Dept Anat, Istanbul, Turkey

[2] Univ Hlth Sci, Umraniye Training & Res Hosp, Istanbul, Turkey

[3] Acibadem Univ, LABGEN, Istanbul, Turkey

[4] Rutgers State Univ, New Jersey Med Sch, Dept Psychiat, New Brunswick, NJ USA

[5] Genz Biotech Inc, Ankara, Turkey

[6] Univ Hlth Sci, Sch Med, Dept Med Biol, Istanbul, Turkey

来源：

GENE REPORTS | 2020年 / 19卷

关键词：

Autism spectrum disorders; Genetic mutation; Monozygotic; Whole exome sequencing; FMN2; KCNQ2; NOTCH3; TMRC6A; SHANK3; SLC6A4; PERVASIVE DEVELOPMENTAL DISORDERS; VARIANTS; RISK; SUSCEPTIBILITY; ASSOCIATION; PREVALENCE;

D O I：

10.1016/j.genrep.2020.100618

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Twin studies provide strong evidence that genetic factors have a major role in the etiology of autism spectrum disorder (ASD) but in most patients the underlying genetic cause of the disease is unknown. Here we used whole-exome sequencing to study genome-wide differences in twins with autism in order to reveal the genetic changes responsible for the etiology of autism. DNA was isolated from peripheral blood samples from six monozygotic twins and one dizygotic twin and analyzed by OneSeq protocol, on an Illumina NextSeq platform. Bioinformatics analyses have revealed 110 disease-related genes, and after further filtering, we have identified 44 single nucleotide polymorphisms (SNPs). Functional network analysis has identified significant associations for 6 different genes, including known ASD candidate genes: FMN2, KCNQ2, NOTCH3, TMRC6A, SHANK3, and SLC6A4. Our results provide an independent evidence for known ASD genes and highlight other genes, which will further improve the understanding of the genetic basis of ASD.

引用

页数：10

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