Combining constitutive materials modeling with atomic force microscopy to understand the mechanical properties of living cells

被引:35
作者
McElfresh, M
Baesu, E
Balhorn, R
Belak, J
Allen, MJ
Rudd, RE
机构
[1] Lawrence Livermore Natl Lab, Mat Res Inst, Livermore, CA 94550 USA
[2] Univ Nebraska, Dept Engn Mech, Lincoln, NE 68588 USA
[3] Biometrol Inc, Alameda, CA 94501 USA
关键词
D O I
10.1073/pnas.082520599
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The goal of this work is to study the properties of living cells and cell membranes by using atomic force microscopy. During atomic force microscopy (AFM) measurement, there is a strong mechanical coupling between the AFM tip and the cell. The purpose of this paper is to present a model of the overall mechanical response of the cell that allows us to separate out the mechanical response of the cell from the local surface interactions we wish to quantify. These local interactions include recognition (or binding) events between molecules bound to an AFM tip (e.g., an antibody) and molecules or receptors on the cell surface (e.g., the respective antigen). The computational model differs from traditional Hertzian contact models by explicitly taking into account the mechanics of the biomembrane and cytoskeleton. The model also accounts for the mechanical response of the living cell during arbitrary deformation. The indentation of a bovine sperm cell is used to test the validity of this model, and further experiments are proposed to fully parameterize the model.
引用
收藏
页码:6493 / 6497
页数:5
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