Immunological responses to antigen B from Echinococcus granulosus cyst fluid in hydatid patients

被引：60

作者：

Ioppolo, S

Notargiacomo, S

Profumo, E

Franchi, C

Ortona, E

Rigano, R

Siracusano, A

机构：

[1] IST SUPER SANITA,IMMUNOL LAB,I-00161 ROME,ITALY

[2] CNR,IST TECNOL BIOMED,ROME,ITALY

[3] UNIV ROMA LA SAPIENZA,CATTEDRA MALATTIE INFETT,ROME,ITALY

来源：

PARASITE IMMUNOLOGY | 1996年 / 18卷 / 11期

关键词：

E-granulosus; hydatid disease; antigen B; ELISA; immunoblotting; PBMC proliferation assay; IgG isotypes;

D O I：

10.1046/j.1365-3024.1996.d01-31.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The immunological reactivity of Echinococcus granulosus antigen B was evaluated in 30 hydatid patients. Antigen B was purified from sheep hydatid cyst fluid by electroelution from a non-reducing SDS-PAGE gel (AgB). In ELISA and immunoblotting (IB), determining antibody production in sera from patients with hydatid disease and with other parasitic infections, purified AgB showed higher specificity than a partially purified antigen named pH(5)PPT (100% vs 83% in pH5PPT-ELISA and 58% in pH(5)PPT-IB). AgB-IB achieved higher sensitivity than AgB-ELISA (80% vs 63%). All AgB-IB positive sera recognized the 12 kDa subunit. Qualitative AgB-IB assessment of IgG isotype responses identified IgG4 as the predominant isotype (87%). The other isotypes showed a lower percentage of positive reactions: IgG1, 33%; IgG2, 21%; and IgG3, 17%. PBMC proliferative assay revealed a cellular response to AgB in 100% of patients' PBMC. These findings confirm antigen B, especially its smallest subunit, as a good diagnostic molecule.

引用

页码：571 / 578

页数：8

共 21 条

[1]

BOMBARDIERI S, 1974, B WORLD HEALTH ORGAN, V51, P525

[2]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[3]

DEFELICE G, 1986, MOL BIOCHEM PARASIT, V20, P133

[4] SEQUENCE HETEROGENEITY OF THE ECHINOCOCCAL ANTIGEN-B [J].