A new Tn1546 type of VanB phenotype-vanA genotype vancomycin-resistant Enterococcus faecium isolates in mainland China

被引：31
作者：
Gu, Li [1 ]
Cao, Bin [2 ]
Liu, Yingmei [2 ]
Guo, Ping [2 ]
Song, Shufan [2 ]
Li, Ran [2 ]
Dai, Huaping [1 ]
Wang, Chen [1 ]
机构：
[1] Capital Med Univ, Beijing Inst Resp Med, Beijing Chaoyang Hosp, Beijing 100020, Peoples R China
[2] Capital Med Univ, Dept Informat Dis & Clin Microbiol, Beijing Chaoyang Hosp, Beijing 100020, Peoples R China
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2009年 / 63卷 / 01期
关键词：
Vancomycin-resistant Enterococcus faecium; VanB phenotype-vanA genotype; Mutilocus sequence typing; Pulsed-field gel electrophoresis; Tn1546-like element; GLYCOPEPTIDE RESISTANCE; ELEMENTS; HUMANS; DIVERSITY; TEICOPLANIN;
D O I：
10.1016/j.diagmicrobio.2008.08.018
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
VanB phenotype-vanA genotype vancomycin-resistant Enterococcus faecium (VREF) has never been reported in mainland China. We investigated the frequency and molecular characteristics of this strain in a Beijing tertiary hospital. Of 23 vanA genotype VREF clinical isolates, 12 (54.3%) were VanB phenotype-vanA genotype. Mutilocus sequence typing (MLST) analysis revealed that all isolates belong to a single clonal complex (CC78), which has been disseminated worldwide. Based on MLST and pulsed-field gel electrophoresis, 23 isolates were polyclonal dissemination in our hospital. Tn1546-like element structure analysis showed that of 12 VanB phenotype-vanA genotype isolates, 5 had complete deletion of vanY and vanZ accompanying insertion of IS1216V in vanX-vanY intergenic region, 5 had ISEfa4 insertion in orf2-vanR intergenic region, a new Tn1546 structure type, and 2 were identical to VanA phenotype-vanA genotype VREF. Data showed that the deletion of vanY and vanZ genes or ISEfa4 insertion in orf2-vanR intergenic region can partly explain the causes of difference between phenotype and genotype. (C) 2008 Elsevier Inc. All rights reserved.
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页码：70 / 75
页数：6
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