The effect of multi-component herbal remedy PERVIVO on cellular immunity and tumor angiogenesis in mice

Herbal extracts from traditional medicine can be formulated to develop novel herbal medicines as potent as synthetic drugs. We investigated properties of multicomponent digestive herbal remedy PERVIVO on innate (non-specific) immunity and adaptive (specific) cellular immunological response in various tests in mice. Previously, we reported stimulatory effect of this remedy on antibody production in mice. In the literature we have found some data about biological activity of the main active, from 27 substances of natural origin, PERVIVO components, in temporary loss of appetite, in gastrointestinal disorders as Crohn's disease and anti-inflammatory, pro-apoptotic and antitumorigenic activities. The aim of our present study was to evaluate the effect of feeding Balb/c mice with PERVIVO on the cellular immune response, and on the growth and vascularity of syngeneic tumor L-1 Sarcoma. The study was performed on 20-22 g of body mass female, F1 hybrid Balb/c x C3H mice (spleen cells recipients in GVH test), and on 8-10-weeks old inbred Balb/c mice, which received orally for 3, 7 or 14 days (40 mu l/mouse/24 hours) PERVIVO, diluted in water 2, 8 and 32 times. Control mice were fed 40 mu l of 10% ethyl alcohol only. Our results indicate that PERVIVO stimulates ex vivo proliferative activity of murine splenocytes, and chemokinetic activity of mouse splenic lymphocytes in tissue culture, and inhibits tumor angio genesis. It has no effect on the number and metabolic activity of blood granulocytes, on ex vivo immunological response of lymphocytes against foreign MHC antigens in local graft-versus-host reaction (lymphocyte-induced angio genesis, LIA), and on the tumor mass.