Differential expression of lactic acid isomers, extracellular matrix metalloproteinase inducer, and matrix metalloproteinase-8 in vaginal fluid from women with vaginal disorders

被引:43
作者
Beghini, J. [1 ,2 ]
Linhares, I. M. [1 ,3 ]
Giraldo, P. C. [2 ]
Ledger, W. J. [1 ]
Witkin, S. S. [1 ]
机构
[1] Weill Cornell Med Coll, Dept Obstet & Gynecol, Div Immunol & Infect Dis, New York, NY 10065 USA
[2] Univ Estadual Campinas, Dept Gynecol & Obstet, Campinas, SP, Brazil
[3] Univ Sao Paulo, Sch Med, Dept Gynecol & Obstet, Sao Paulo, Brazil
基金
巴西圣保罗研究基金会;
关键词
Bacterial vaginosis; cytolytic vaginosis; d-lactic acid; extracellular matrix metalloproteinase inducer; l-lactic acid; matrix metalloproteinase-8; vulvovaginal candidiasis; BACTERIAL VAGINOSIS; CD147;
D O I
10.1111/1471-0528.13072
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
ObjectiveDo metabolites in vaginal samples vary between women with different vaginal disorders. DesignCross-sectional study. SettingCampinas, Brazil. SampleSeventy-seven women (39.9%) with no vaginal disorder, 52 women (26.9%) with vulvovaginal candidiasis (VVC), 43 women (22.3%) with bacterial vaginosis (BV), and 21 women (10.9%) with cytolytic vaginosis (CTV). MethodConcentrations of d- and l-lactic acid, extracellular matrix metalloproteinase inducer (EMMPRIN), and matrix metalloproteinase-8 (MMP-8), and the influence of Candida albicans on EMMPRIN production by cultured vaginal epithelial cells, were determined by enzyme-linked immunosorbent assay (ELISA). Associations were determined by the Mann-Whitney U-test and by Spearman's rank correlation test. Main outcome measuresMetabolite levels and their correlation with diagnoses. ResultsVaginal concentrations of d- and l-lactic acid were reduced from control levels in BV (P<0.0001); l-lactic acid levels were elevated in CTV (P=0.0116). EMMPRIN and MMP-8 concentrations were elevated in VVC (P<0.0001). EMMPRIN and l-lactic acid concentrations (P0.008), but not EMMPRIN and d-lactic acid, were correlated in all groups. EMMPRIN also increased in proportion with the ratio of l- to d-lactic acid in controls and in women with BV (P0.009). Concentrations of EMMPRIN and MMP-8 were correlated in controls and women with VVC (P0.0002). Candida albicans induced EMMPRIN release from vaginal epithelial cells. ConclusionsVaginal secretions from women with BV are deficient in d- and l-lactic acid, women with VVC have elevated EMMPRIN and MMP-8 levels, and women with CTV have elevated l-lactic acid levels. These deviations may contribute to the clinical signs, symptoms, and sequelae that are characteristic of these disorders.
引用
收藏
页码:1580 / 1585
页数:6
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