Functional CD1a is stabilized by exogenous lipids

被引:53
|
作者
Manolova, Vania
Kistowska, Magdalena
Paoletti, Samantha
Baltariu, Gabriel M.
Bausinger, Huguette
Hanau, Daniel
Mori, Lucia
De Libero, Gennaro
机构
[1] Univ Basel Hosp, Dept Res, CH-4031 Basel, Switzerland
[2] Univ Strasbourg, Etab Francais Sang Alsace, INSERM, U725, Strasbourg, France
关键词
antigen loading; antigen presentation; CD1; lipid recognition;
D O I
10.1002/eji.200535544
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Self-glycosphingolipids bind to surface CD1 molecules and are readily displaced by other CD1 ligands. This capacity to exchange antigens at the cell surface is not common to other antigen-presenting molecules and its physiological importance is unclear. Here we show that a large pool of cell-surface CD1a, but not CD1b molecules, is stabilized by exogenous lipids present in serum. Under serum deprivation CD1a molecules are altered and functionally inactive, as they are unable to present lipid antigens to T cells. Glycosphingolipids and phospholipids bind to, and restore functionality to CD1a without the contribution of newly synthesized and recycling CD1a molecules. The dependence of CD1a stability on exogenous lipids is not related to its intracellular traffic and rather to its antigen-binding pockets. These results indicate a functional dichotomy between CD1a and CD1b molecules and provide new information on how the lipid antigenic repertoire is immunologically sampled.
引用
收藏
页码:1083 / 1092
页数:10
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