Stability of Immobilized Candida sp 99-125 Lipase for Biodiesel Production

被引：22

作者：

Lu, Jike ^{[1
,2
]}

Deng, Li ^{[1
]}

Nie, Kaili ^{[1
]}

Wang, Fang ^{[1
]}

Tan, Tianwei ^{[1
]}

机构：

[1] Beijing Univ Chem Technol, Beijing Bioproc Key Lab, Coll Life Sci & Technol, Beijing 100029, Peoples R China

[2] Zhengzhou Univ, Dept Bioengn, Zhengzhou, Peoples R China

来源：

CHEMICAL ENGINEERING & TECHNOLOGY | 2012年 / 35卷 / 12期

基金：

国家高技术研究发展计划(863计划);

关键词：

Candida sp; 99-125; Biodiesel; Immobilized lipase; Stability; FUEL PRODUCTION; CATALYZED METHANOLYSIS; GLYCEROL TRIOLEATE; ANTARCTICA LIPASE; ORGANIC-SOLVENT; OIL; WATER; PRETREATMENT; SP-99-125; IMPROVE;

D O I：

10.1002/ceat.201200254

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

The stability of the immobilized lipase from Candida sp. 99-125 during biodiesel production was investigated. The lipase was separately incubated in the presence of various reaction components such as soybean oil, oleic acid methyl ester, n-hexane, water, methanol, and glycerol, or the lipase was stored at 60, 80, 100 and 120 degrees C. Thereafter the residual lipase activity was determined by methanolysis reaction. The results showed that the lipase was rather stable in the reaction media, except for methanol and glycerol. The stability study performed in a reciprocal shaker indicated that enzyme desorption from the immobilized lipase mainly contributed to the lipase inactivation in the water system. So the methanol and glycerol contents should be controlled more precisely to avoid lipase inactivation, and the immobilization method should be improved with regard to lipase desorption.

引用

页码：2120 / 2124

页数：5

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