Preparation and properties of a novel drug delivery system with both magnetic and biomolecular targeting

被引：81

作者：

Yang, Yong ^{[1
]}

Jiang, Ji-Sen ^{[1
]}

Du, Bing ^{[2
]}

Gan, Zhi-Feng ^{[1
]}

Qian, Min ^{[2
]}

Zhang, Ping ^{[2
]}

机构：

[1] E China Normal Univ, Dept Phys, Ctr Funct Nanomat & Devices, Shanghai 200062, Peoples R China

[2] E China Normal Univ, Sch Life Sci, Shanghai 200062, Peoples R China

来源：

JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE | 2009年 / 20卷 / 01期

关键词：

IRON-OXIDE NANOPARTICLES; IN-VIVO; PHAGE DISPLAY; PEPTIDE; TUMORS; PH; MICROSPHERES; ADRIAMYCIN; FERRITES; RELEASE;

D O I：

10.1007/s10856-008-3577-0

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

By loading doxorubicin (DOX) on 5-carboxyl-fluorescein (FAM) labeled AGKGTPSLETTP peptide (A54) coupled starch-coated iron oxide nanoparticles (SIONs), we prepared a novel aqueous drug delivery system with both magnetic and biomolecular targeting, which was specific to human hepatocellular carcinoma cell line BEL-7402. The saturated extent of adsorption reached 2.0 mg DOX/mg A54-SIONs at 28A degrees C, which provided a rather high dose of DOX loading for application. Tests in vitro demonstrated the specificity of DOX-loaded A54-SIONs to BEL-7402 cells. The microscopy images proved that DOX-loaded A54-SIONs were successfully targeted to tumor tissue of nude mice with an external magnetic field in vivo. MTT assay showed higher cytostatic effect of DOX-loaded A54-SIONs to hepatocellular carcinoma cells BEL-7402 than that of DOX-loaded SIONs.

引用

页码：301 / 307

页数：7

共 28 条

[1] Novel bioactive and stable neurotensin peptide analogues capable of delivering radiopharmaceuticals and molecular beacons to tumors [J].

Achilefu, S ;

Srinivasan, A ;

Schmidt, MA ;

Jimenez, HN ;

Bugaj, JE ;

Erion, JL .

JOURNAL OF MEDICINAL CHEMISTRY, 2003, 46 (15) :3403-3411

[2] Therapeutic cancer targeting peptides [J].

Aina, OH ;

Sroka, TC ;

Chen, ML ;

Lam, KS .

BIOPOLYMERS, 2002, 66 (03) :184-199

[3] Nanocrystal targeting in vivo [J].

Åkerman, ME ;

Chan, WCW ;

Laakkonen, P ;

Bhatia, SN ;

Ruoslahti, E .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (20) :12617-12621

[4] Diacyllipid micelle-based nanocarrier for magnetically guided delivery of drugs in photodynamic therapy [J].

Cinteza, Ludmila O. ;

Ohulchanskyy, Tymish Y. ;

Sahoo, Yudhisthira ;

Bergey, Earl J. ;

Pandey, Ravindra K. ;

Prasad, Paras N. .

MOLECULAR PHARMACEUTICS, 2006, 3 (04) :415-423

[5] EXTRACELLULAR PH DISTRIBUTION IN HUMAN TUMORS [J].

ENGIN, K ;

LEEPER, DB ;

CATER, JR ;

THISTLETHWAITE, AJ ;

TUPCHONG, L ;

MCFARLANE, JD .

INTERNATIONAL JOURNAL OF HYPERTHERMIA, 1995, 11 (02) :211-216

[6]

Gan ZF, 2005, PROG CHEM, V17, P978

[7] Immobilization of homing peptide on magnetite nanoparticles and its specificity in vitro [J].

Gan, Zhi-Feng ;

Jiang, Ji-Sen ;

Yang, Yong ;

Du, Bing ;

Qian, Min ;

Zhang, Ping .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2008, 84A (01) :10-18

[8] The synthesis of a prodrug of doxorubicin designed to provide reduced systemic toxicity and greater target efficacy [J].

Garsky, VM ;

Lumma, PK ;

Feng, DM ;

Wai, J ;

Ramjit, HG ;

Sardana, MK ;

Oliff, A ;

Jones, RE ;

DeFeo-Jones, D ;

Freidinger, RM .

JOURNAL OF MEDICINAL CHEMISTRY, 2001, 44 (24) :4216-4224

[9] Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications [J].

Gupta, AK ;

Gupta, M .

BIOMATERIALS, 2005, 26 (18) :3995-4021

[10] Iron oxide nanoparticles for sustained delivery of anticancer agents [J].

Jain, Tapan K. ;

Morales, Marco A. ;

Sahoo, Sanjeeb K. ;

Leslie-Pelecky, Diandra L. ;

Labhasetwar, Vinod .

MOLECULAR PHARMACEUTICS, 2005, 2 (03) :194-205

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