Isolation and genetic determination of a Saccharomyces cerevisiae mutant that produces an endo-polygalacturonase

被引：7
作者：
Hirose, N
Fujii, M
Kishida, M
Kawasaki, H
Sakai, T
机构：
[1] Osaka Prefecture Univ, Coll Agr, Dept Appl Biochem, Sakai, Osaka 5998531, Japan
[2] Kinki Univ, Fac Agr, Dept Food Sci, Nara 6310052, Japan
来源：
JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 1999年 / 87卷 / 05期
关键词：
Saccharomyces cerevisiae; polygalacturonase; mutant; silent gene; genetic determination; repressor;
D O I：
10.1016/S1389-1723(99)80120-0
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A Saccharomyces cerevisiae mutant that produces an endo-polygalacturonase (PGase) was isolated. The PGase gene was revealed to be located on chromosome X in both the mutant and its parental strain. The 5'-upstream region of the PGase gene in the mutant was entirely identical with that of its parent. Crossing of the mutant with a PGase-negative strain showed that the phenotype of PGase production was recessive. These results suggest that the mutant is rendered defective in a transacting factor that normally represses the expression of the PGase gene.
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收藏
页码：594 / 597
页数：4
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