Immunostimulatory effect of Antrodia camphorata extract on functional maturation of dendritic cells

被引：35

作者：

Lu, Mei-Chin ^{[1
]}

Hwang, Shiuh-Lin ^{[2
]}

Chang, Fang-Rong ^{[1
,3
]}

Chen, Yung-Husan ^{[1
]}

Chang, Tun-Tschu ^{[4
]}

Hung, Chih-Sheng ^{[1
]}

Wang, Chun-Li ^{[5
]}

Chu, Yao-Hsiang ^{[5
]}

Pan, Shu-Hua ^{[6
]}

Wu, Yang-Chang ^{[1
,3
]}

机构：

[1] Kaohsiung Med Univ, Sch Pharm, Grad Inst Nat Prod, Kaohsiung 807, Taiwan

[2] Kaohsiung Med Univ Hosp, Dept Surg, Div Neurosurg, Kaohsiung 807, Taiwan

[3] Kaohsiung Med Univ, Ctr Excellence Environm Med, Kaohsiung 807, Taiwan

[4] Taiwan Forestry Res Inst, Div Forest Protect, Taipei 100, Taiwan

[5] Natl Pingtung Univ Sci & Technol, Dept Plant Ind, Pingtung 912, Taiwan

[6] Kaohsiung Med Univ Hosp, Dept Nursing, Kaohsiung 807, Taiwan

来源：

FOOD CHEMISTRY | 2009年 / 113卷 / 04期

关键词：

Antrodia camphorata; Polysaccharides; Dendritic cells; MAPKs; PI3K/Akt; KAPPA-B; SUBMERGED CULTURE; AGARICUS-BLAZEI; IMMUNE-RESPONSE; IN-VIVO; POLYSACCHARIDES; INFLAMMATION; INHIBITION; MODULATION; EXPRESSION;

D O I：

10.1016/j.foodchem.2008.08.089

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Dendritic cells (DCs) are now recognised as the most potent professional antigen presenting cells (APCs) involved in initiating primary immune responses. The medicinal Mushroom Antrodia camphorata (AC) is one of the Most Popular chemopreventive mushrooms in Taiwan. Polysaccharides of mushroom products are among emerging new agents that activate maturation and functions of DCs. The aim of this study is to investigate the immunomodulating activity of A. camphorata extract (ACW) on functional maturation of DCs. Compared with lipopolysaccharides (LPS), ACW effectively promoted the functional Maturation of DCs in the expression of phenotypic characteristics and IL-12 production and chemotaxic activity. Moreover, ACW increased the phosphorylation of Akt, p38, and JNK/MAPKs in DCs. Specific inhibitors, SB203580 and LY94002, significantly blocked the ACW-induced up-regulation Of costimulatory factor expression, IL-12 production. These findings suggest that ACW is a potent adjuvant for cancer immunotherapy and promotes Th1 immune responses. (c) 2008 Elsevier Ltd. All rights reserved.

引用

页码：1049 / 1057

页数：9

共 29 条

[1] Controllers of the immune system and a new promise for immunotherapy
Banchereau, J
Paczesny, S
Blanco, P
Bennett, L
Pascual, V
Fay, J
Palucka, AK
[J]. IMMUNE MECHANISMS AND DISEASE, 2003, 987 : 180 - 187
[2] Response of human dendritic cells to different immunomodulatory polysaccharides derived from mushroom and barley
Chan, Wing Keung
Law, Helen Ka Wai
Lin, Zhi-Bin
Lau, Yu Lung
Chan, Godfrey Chi-Fung
[J]. INTERNATIONAL IMMUNOLOGY, 2007, 19 (07) : 891 - 899
[3] Chemical characterization and anti-inflammatory effect of polysaccharides fractionated from submerge-cultured Antrodia camphorata mycelia
Chen, Chin-Chu
Liu, Yi-Wen
Ker, Yaw-Bee
Wu, Yen-Yin
Lai, Eric Y.
Chyau, Charng-Cherng
Hseu, Tzong-Hsiung
Peng, Robert Y.
[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2007, 55 (13) : 5007 - 5012
[4] Regulation of the lifespan in dendritic cell subsets
Chen, Min
Huang, Li
Shabier, Zainuer
Wang, Jin
[J]. MOLECULAR IMMUNOLOGY, 2007, 44 (10) : 2558 - 2565
[5] TLR ligands that stimulate the metabolism of vitamin D3 in activated murine dendritic cells can function as effective mucosal adjuvants to subcutaneously administered vaccines
Enioutina, Elena Y.
Bareyan, Diana
Daynes, Raymond A.
[J]. VACCINE, 2008, 26 (05) : 601 - 613
[6] Quinacrine inhibits the epidermal dendritic cell migration initiating T cell-mediated skin inflammation
Gorbachev, Anton V.
Gasparian, Alexander V.
Gurova, Katerina V.
Gudkov, Andrei V.
Fairchild, Robert L.
[J]. EUROPEAN JOURNAL OF IMMUNOLOGY, 2007, 37 (08) : 2257 - 2267
[7] The pathophysiology of mitochondrial cell death
Green, DR
Kroemer, G
[J]. SCIENCE, 2004, 305 (5684) : 626 - 629
[8] Dendritic cells: Unique leukocyte populations which control the primary immune response
Hart, DNJ
[J]. BLOOD, 1997, 90 (09) : 3245 - 3287
[9] Anti-inflammatory potential of Antrodia Camphorata through inhibition of NOS, COX-2 and cytokines via the NF-κB pathway
Hseu, YC
Wu, FY
Wu, JJ
Chen, JY
Chang, WH
Lu, FJ
Lai, YC
Yang, HL
[J]. INTERNATIONAL IMMUNOPHARMACOLOGY, 2005, 5 (13-14) : 1914 - 1925
[10] TRANCE, a tumor necrosis factor family member, enhances the longevity and adjuvant properties of dendritic cells in vivo
Josien, R
Li, HL
Ingulli, E
Sarma, S
Wong, BR
Vologodskaia, M
Steinman, RM
Choi, Y
[J]. JOURNAL OF EXPERIMENTAL MEDICINE, 2000, 191 (03) : 495 - 501

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