Enhanced cellulose-hydrogen production from corn stalk by lesser panda manure

被引:77
作者
Fan, Yao-Ting [1 ]
Xing, Yan [1 ]
Ma, Hong-Cui [1 ]
Pan, Chun-Mei [1 ]
Hou, Hong-Wei [1 ]
机构
[1] Zhengzhou Univ, Dept Chem, Zhengzhou 450052, Henan, Peoples R China
基金
中国国家自然科学基金;
关键词
Clostridium sp; Lesser panda manure; Bio-pretreatment; Corn stalk; Cellulose-H-2;
D O I
10.1016/j.ijhydene.2008.08.005
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
in this study, the predominant H-2-producing bacterial strain was isolated from a bio-reactor fed with lesser panda manure, and identified as Clostridium sp. based on 16S rDNA gene analysis. The bio-pretreatment of corn stalk was found to be most effective at ambient temperature of 25 degrees C, microbe additive of 7.5 g/kg with saccharification period of 15 days under the anaerobic condition. The maximum H-2-production yield (176 ml H-2/g-TS) and rate (18ml H-2/g-TSh(-1)) were achieved at operating pH 5.5, 36 degrees C and substrate concentration of 15 g/L. The H-2 content in the biogas was 44.3-57.2% (v/v) and there was no significant methane gas observed. During the optimal period of H-2 production, the ORP values stayed in the lower level ranging from -445 mV to -455 mV. The results indicated that the effluent was composed mostly of butyric acid and acetic acid, which accounted for 70-80% of total VFAs, followed by small amounts of butanol and propionate. (C) 2008 International Association for Hydrogen Energy. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:6058 / 6065
页数:8
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