Enzymatic Biosensors with Integrated Thin Film a-Si:H Photodiodes

被引：2

作者：

Pereira, A. T. ^{[1
,2
]}

Chu, V. ^{[1
]}

Prazeres, D. M. F. ^{[2
,3
]}

Conde, J. P. ^{[1
,3
]}

机构：

[1] INESC Microsistemas & Nanotecnol, Rua Alves Redol 9, Lisbon, Portugal

[2] Inst Super Tecn, Inst Biotechnol & Bioengn, Ctr Engn Biol Quim, Lisbon, Portugal

[3] Inst Super Tecn, Dept Chem & Biol Engn, Lisbon, Portugal

来源：

AMORPHOUS AND POLYCRYSTALLINE THIN FILM SILICON SCIENCE AND TECHNOLOGY - 2009, VOL 1153 | 2009年 / 1153卷

关键词：

AMORPHOUS-SILICON PHOTODIODE;

D O I：

10.1557/PROC-1153-A08-04

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

A microfabricated amorphous silicon photodiode is used to detect chemiluminescent and colorimetric horseradish peroxidase (HRP) enzymatic reactions. Detections limits of 1 nM and 1 pM of HRP are obtained for chemiluminescent and colorimetric measurements, respectively, with the reactions carried out in solution volume of 50 mu L in polystyrene microwells. Surface-adsorbed HRP can be detected with a limit of 1 fmol.cm(-2) by both detection methods. Immunoassays were performed using HRP-labeled antibodies and the detection of specific antibody-antigen molecular recognition is demonstrated both in the plastic well and inside a microfluidic channel. The application of the a-Si:H/HRP system is extended by coupling HRP with oxidase enzyme systems for glucose detection and a sensitivity of 0.1 mmol/L was achieved.

引用

页数：6

共 5 条

[1] Azevedo AM, 2003, BIOTECHNOL ANN REV, V9, P199, DOI 10.1016/S1387-2656(03)09003-3
[2] Colorimetric detection of molecular recognition reactions with an enzyme biolabel using a thin-film amorphous silicon photodiode on a glass substrate
Gouvea, A.
Pereira, A. T.
Pimentel, A. C.
Prazeres, D. M. F.
Chu, V.
Conde, J. P.
[J]. SENSORS AND ACTUATORS B-CHEMICAL, 2008, 135 (01) : 102 - 107
[3] Pereira A. T., IEEE SENSOR IN PRESS
[4] Detection of chemiluminescence using an amorphous silicon photodiode
Pimentel, Alexandra C.
Pereira, A. T.
Chu, V.
Prazeres, D. M. F.
Conde, J. P.
[J]. IEEE SENSORS JOURNAL, 2007, 7 (3-4) : 415 - 416
[5] Street R. A., 2005, HYDROGENATED AMORPHO

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