A photocleavable surfactant for top-down proteomics

被引：83

作者：

Brown, Kyle A. ^{[1
]}

Chen, Bifan ^{[1
]}

Guardado-Alvarez, Tania M. ^{[1
]}

Lin, Ziqing ^{[2
,3
]}

Hwang, Leekyoung ^{[1
]}

Ayaz-Guner, Serife ^{[2
]}

Jin, Song ^{[1
]}

Ge, Ying ^{[1
,2
,3
]}

机构：

[1] Univ Wisconsin, Dept Chem, 1101 Univ Ave, Madison, WI 53706 USA

[2] Univ Wisconsin, Sch Med & Publ Hlth, Dept Cell & Regenerat Biol, Madison, WI 53706 USA

[3] Univ Wisconsin, Sch Med & Publ Hlth, Human Prote Program, Madison, WI 53706 USA

来源：

NATURE METHODS | 2019年 / 16卷 / 05期

基金：

美国国家卫生研究院;

关键词：

SPECTROMETRY-COMPATIBLE SURFACTANT; MASS-SPECTROMETRY; MEMBRANE-PROTEINS; SOFTWARE TOOL;

D O I：

10.1038/s41592-019-0391-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the identification of a photocleavable anionic surfactant, 4-hexylphenylazosulfonate (Azo), which can be rapidly degraded by ultraviolet irradiation, for top-down proteomics. Azo can effectively solubilize proteins with performance comparable to that of sodium dodecyl sulfate (SDS) and is compatible with mass spectrometry. Azo-aided top-down proteomics enables the solubilization of membrane proteins for comprehensive characterization of post-translational modifications. Moreover, Azo is simple to synthesize and can be used as a general SDS replacement in SDS-polyacrylamide gel electrophoresis.

引用

页码：417 / +

页数：7

共 33 条

[1]

Aebersold R, 2018, NAT CHEM BIOL, V14, P206, DOI [10.1038/NCHEMBIO.2576, 10.1038/nchembio.2576]

[2]

Apweiler R, 2004, NUCLEIC ACIDS RES, V32, pD115, DOI [10.1093/nar/gkw1099, 10.1093/nar/gkh131]

[3] Gene Ontology: tool for the unification of biology [J].

Ashburner, M ;

Ball, CA ;

Blake, JA ;

Botstein, D ;

Butler, H ;

Cherry, JM ;

Davis, AP ;

Dolinski, K ;

Dwight, SS ;

Eppig, JT ;

Harris, MA ;

Hill, DP ;

Issel-Tarver, L ;

Kasarskis, A ;

Lewis, S ;

Matese, JC ;

Richardson, JE ;

Ringwald, M ;

Rubin, GM ;

Sherlock, G .

NATURE GENETICS, 2000, 25 (01) :25-29

[4] Advances in the Mass Spectrometry of Membrane Proteins: From Individual Proteins to Intact Complexes [J].

Barrera, Nelson P. ;

Robinson, Carol V. .

ANNUAL REVIEW OF BIOCHEMISTRY, VOL 80, 2011, 80 :247-271

[5] Photoresponsive surfactants in microgel dispersions [J].

Bradley, M ;

Vincent, B ;

Warren, N ;

Eastoe, J ;

Vesperinas, A .

LANGMUIR, 2006, 22 (01) :101-105

[6] Top-Down Proteomics of Large Proteins up to 223 kDa Enabled by Serial Size Exclusion Chromatography Strategy [J].

Cai, Wenxuan ;

Tucholski, Trisha ;

Chen, Bifan ;

Alpert, Andrew J. ;

McIlwain, Sean ;

Kohmoto, Takushi ;

Jin, Song ;

Ge, Ying .

ANALYTICAL CHEMISTRY, 2017, 89 (10) :5467-5475

[7] Top-down Proteomics: Technology Advancements and Applications to Heart Diseases [J].

Cai, Wenxuan ;

Tucholski, Trisha M. ;

Gregorich, Zachery R. ;

Ge, Ying .

EXPERT REVIEW OF PROTEOMICS, 2016, 13 (08) :717-730

[8] MASH Suite Pro: A Comprehensive Software Tool for Top-Down Proteomics [J].

Cai, Wenxuan ;

Guner, Huseyin ;

Gregorich, Zachery R. ;

Chen, Albert J. ;

Ayaz-Guner, Serife ;

Peng, Ying ;

Valeja, Santosh G. ;

Liu, Xiaowen ;

Ge, Ying .

MOLECULAR & CELLULAR PROTEOMICS, 2016, 15 (02) :703-714

[9] New Mass-Spectrometry-Compatible Degradable Surfactant for Tissue Proteomics [J].

Chang, Ying-Hua ;

Gregorich, Zachery R. ;

Chen, Albert J. ;

Hwang, Leekyoung ;

Guner, Huseyin ;

Yu, Deyang ;

Zhang, Jianyi ;

Ge, Ying .

JOURNAL OF PROTEOME RESEARCH, 2015, 14 (03) :1587-1599

[10] Top-Down Proteomics: Ready for Prime Time? [J].

Chen, Bifan ;

Brown, Kyle A. ;

Lin, Ziqing ;

Ge, Ying .

ANALYTICAL CHEMISTRY, 2018, 90 (01) :110-127

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