Modulation of rat cecal microbiota by administration of raffinose and encapsulated Bifidobacterium breve

被引:44
作者
Dinoto, A
Suksomcheep, A
Ishizuka, S
Kimura, H
Hanada, S
Kamagata, Y
Asano, K
Tomita, F
Yokota, A
机构
[1] Hokkaido Univ, Grad Sch Agr, Lab Microbial Resources & Ecol, Kita Ku, Sapporo, Hokkaido 0608589, Japan
[2] Hokkaido Univ, Grad Sch Agr, Lab Nutr Biochem, Kita Ku, Sapporo, Hokkaido 0608589, Japan
[3] Hokkaido Univ, Grad Sch Agr, Appl Microbiol Lab, Kita Ku, Sapporo, Hokkaido 0608589, Japan
[4] Hokkaido Collaborat Ctr, No Advancement Ctr Sci & Technol, Kita Ku, Sapporo, Hokkaido 0010021, Japan
[5] Natl Inst Adv Ind Sci & Technol AIST, Inst Biol Resources & Funct, Tsukuba, Ibaraki 3058566, Japan
关键词
D O I
10.1128/AEM.72.1.784-792.2006
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
To investigate the effects of administration of raffinose and encapsulated Bifidobacterium breve JCM 1192(T) cells on the rat cecal microbiota, in a preclinical synbiotic study groups of male WKAH/Hkm Sic rats were fed for 3 weeks with four different test diets: basal diet (group BD), basal diet supplemented with raffinose (group RAF), basal diet supplemented with encapsulated B. breve (group CB), and basal diet supplemented with both raffinose and encapsulated B. breve (group RCB). The bacterial populations in cecal samples were determined by fluorescence in situ hybridization (FISH) and terminal restriction fragment length polymorphism (T-RFLP). B. breve cells were detected only in the RCB group and accounted for about 6.3% of the total cells as determined by FISH analysis. B. breve was also detected only in the RCB group by T-RFLP analysis. This was in contrast to the CB group, in which no B. breve signals were detected by either FISH or T-RFLP. Increases in the sizes of the populations of Bifidobacterium animalis, a Bifidobacterium indigenous to the rat, were observed in the RAF and RCB groups. Principal-component analysis of T-RFLP results revealed significant alterations in the bacterial populations of rats in the RAF and RCB groups; the population in the CB group was similar to that in the control group (group BID). To the best of our knowledge, these results provide the first clear picture of the changes in the rat cecal microbiota in response to synbiotic administration.
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收藏
页码:784 / 792
页数:9
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