Shrinkage of cutaneous specimens: formalin or other factors involved?

被引:109
作者
Kerns, Mary Jo J. [1 ]
Darst, Marc A. [1 ]
Olsen, Thomas G. [1 ]
Fenster, Mark [1 ]
Hall, Philip [1 ]
Grevey, Scott [1 ]
机构
[1] Wright State Univ, Dept Dermatol, Dayton, OH 45435 USA
关键词
D O I
10.1111/j.1600-0560.2007.00943.x
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Background: Shrinkage of cutaneous tissue during processing is a source of controversy. This study was designed to prospectively determine tissue shrinkage at two intervals: 1 min after excision and after 24 to 48 h of formalin fixation. Secondarily, gender, age, site, prior biopsy scar and solar elastosis were evaluated with respect to shrinkage. Methods: Ninety-seven cutaneous specimens were measured prior to excision, 1 min after removal and after 24 to 48 h of formalin fixation. Width of prior biopsy scar, damage to elastic fibers and solar elastosis were subjectively quantified. Results: Significant tissue shrinkage occurred immediately after excision, prior to formalin fixation. Mean shrinkage (95% confidence interval): length 20.66% +/- 2.15% and width 11.79% +/- 2.35%. Range of shrinkage: length 0 to 41.18% and width -18.75% (indicating expansion) to 37.50%. Patient age was significant; shrinkage decreased 0.3% per year of increasing age. Site was less significant; trunk excisions measured 5% greater shrinkage than head/neck excisions. As solar elastosis increased, shrinkage decreased. Conclusions: Cutaneous tissue shrinkage following excision is primarily because of intrinsic tissue contractility. Increasing patient age and solar elastosis correlate with less shrinkage. The clinicians and dermatopathologists must be cognizant of the expected shrinkage of submitted specimens for settling discrepancies within the medical record.
引用
收藏
页码:1093 / 1096
页数:4
相关论文
共 12 条
[1]   CERVICAL TISSUE SHRINKAGE BY FORMALDEHYDE FIXATION, PARAFFIN WAX EMBEDDING, SECTION CUTTING AND MOUNTING [J].
BOONSTRA, H ;
OOSTERHUIS, JW ;
OOSTERHUIS, AM ;
FLEUREN, GJ .
VIRCHOWS ARCHIV A-PATHOLOGICAL ANATOMY AND HISTOPATHOLOGY, 1983, 402 (02) :195-201
[2]   Intrinsic aging vs. photoaging: a comparative histopathological, immunohistochemical, and ultrastructural study of skin [J].
El-Domyati, M ;
Attia, S ;
Saleh, F ;
Brown, D ;
Birk, DE ;
Gasparro, F ;
Ahmad, H ;
Uitto, J .
EXPERIMENTAL DERMATOLOGY, 2002, 11 (05) :398-405
[3]   Predictable tissue shrinkage during frozen section histopathologic processing for Mohs micrographic surgery [J].
Gardner, ES ;
Sumner, WT ;
Cook, JL .
DERMATOLOGIC SURGERY, 2001, 27 (09) :813-818
[4]  
Goldstein NS, 1999, AM J CLIN PATHOL, V111, P349
[5]   DETERMINATION OF PREEXCISION SURGICAL MARGINS OF MELANOMAS FROM FIXED-TISSUE SPECIMENS [J].
GOLOMB, FM ;
DOYLE, JP ;
GRIN, CM ;
KOPF, AW ;
SILVERMAN, MK ;
LEVENSTEIN, MJ .
PLASTIC AND RECONSTRUCTIVE SURGERY, 1991, 88 (05) :804-809
[6]   RELATION BETWEEN SIZE OF SKIN EXCISION, WOUND, AND SPECIMEN [J].
HUDSONPEACOCK, MJ ;
MATTHEWS, JNS ;
LAWRENCE, CM .
JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY, 1995, 32 (06) :1010-1015
[7]   THE INFLUENCE OF TISSUE PROCESSING ON QUANTITATIVE HISTOPATHOLOGY IN BREAST-CANCER [J].
LADEKARL, M .
JOURNAL OF MICROSCOPY-OXFORD, 1994, 174 :93-100
[8]  
LUM H, 1985, AM REV RESPIR DIS, V132, P1078
[9]  
MA GW, 2004, ZHENG, V23, P193
[10]   The shrinkage of the human brain stem during formalin fixation and embedding in paraffin [J].
Quester, R ;
Schroder, R .
JOURNAL OF NEUROSCIENCE METHODS, 1997, 75 (01) :81-89