Probing force-induced unfolding intermediates of a single staphylococcal nuclease molecule and the effect of ligand binding

被引：7

作者：

Ishii, Takaaki ^{[1
]}

Murayama, Yoshihiro ^{[2
]}

Katano, Atsuto ^{[1
,2
,3
,4
,5
,6
]}

Maki, Kosuke ^{[3
]}

Kuwajima, Kunihiro ^{[4
,5
,6
]}

Sano, Masaki ^{[1
]}

机构：

[1] Univ Tokyo, Dept Phys, Grad Sch Sci, Bunkyo Ku, Tokyo 1130033, Japan

[2] Tokyo Univ Agr & Technol, Dept Appl Phys, Grad Sch Engn, Koganei, Tokyo 1848588, Japan

[3] Nagoya Univ, Grad Sch Sci, Chikusa Ku, Nagoya, Aichi 4648602, Japan

[4] Natl Inst Nat Sci, Okazaki Inst Integrat Biosci, Okazaki, Aichi 4448787, Japan

[5] Natl Inst Nat Sci, Inst Mol Sci, Okazaki, Aichi 4448787, Japan

[6] Grad Univ Adv Studies SOKENDAI, Dept Funct Mol Sci, Sch Phys Sci, Okazaki, Aichi 4448787, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 375卷 / 04期

关键词：

Atomic force microscope (AFM); Staphylococcal nuclease (SNase); Mechanical unfolding; Unfolding intermediate; Ligand-binding complex;

D O I：

10.1016/j.bbrc.2008.08.073

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Single-molecule manipulation techniques have given experimental access to unfolding intermediates of proteins that are inaccessible in conventional experiments. A detailed characterization of the intermediates is a challenging problem that provides new possibilities for directly probing the energy landscape of proteins. We investigated single-molecule mechanical unfolding of a small globular protein, staphylococcal nuclease (SNase), using atomic force microscopy. The unfolding trajectories of the protein displayed sub-molecular and stochastic behavior with typical lengths corresponding to the size of the unfolded substructures. Our results support the view that the single protein unfolds along multiple pathways as suggested in recent theoretical studies. Moreover, we found the drastic change, caused by the ligand and inhibitor bindings, in the mechanical unfolding dynamics. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：586 / 591

页数：6

共 32 条

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