MonoRes: Automatic and Accurate Estimation of Local Resolution for Electron Microscopy Maps

被引：143

作者：

Luis Vilas, Jose ^{[1
]}

Gomez-Blanco, Josue ^{[1
]}

Conesa, Pablo ^{[1
]}

Melero, Roberto ^{[1
]}

iguel de la Rosa-Trevin, Jose ^{[2
]}

Oton, Joaquin ^{[1
]}

Cuenca, Jesus ^{[1
]}

Marabini, Roberto ^{[3
]}

Maria Carazo, Jose ^{[1
]}

Vargas, Javier ^{[4
]}

Sorzano, Carlos Oscar S. ^{[1
,5
]}

机构：

[1] CSIC, Natl Ctr Biotechnol, Biocomp Unit, Darwin 3,Campus Univ Autonoma, E-28049 Madrid, Spain

[2] Stockholm Univ, Sci Life Lab, Stockholm, Sweden

[3] Univ Autonoma Madrid, Escuela Politecn Super, E-28049 Madrid, Spain

[4] McGill Univ, Dept Anat & Cell Biol, Univ St Strathcona Anat Bldg 3640, Montreal, PQ, Canada

[5] Univ San Pablo CEU, Dept Engn Elect & Telecommun Syst, Campus Urbanizac Monteprincipe, Madrid 28668, Spain

来源：

STRUCTURE | 2018年 / 26卷 / 02期

关键词：

CRYO-EM STRUCTURE; SIGNAL; TRANSFORM; RECONSTRUCTIONS; DEMODULATION;

D O I：

10.1016/j.str.2017.12.018

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Since the beginning of electron microscopy, resolution has been a critical parameter. In this article, we propose a fully automatic, accurate method for determining the local resolution of a 3D map (MonoRes). The foundation of this algorithm is an extension of the concept of analytic signal, termed monogenic signal. The map is filtered at different frequencies and the amplitude of the monogenic signal is calculated, after which a criterion is applied to determine the resolution at each voxel. MonoRes is fully automatic without compulsory user parameters, with great accuracy in all tests, and is computationally more rapid than existing methods in the field. In addition, MonoRes offers the option of local filtering of the original map based on the calculated local resolution.

引用

页码：337 / +

页数：12

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