Clostridioides difficile from Brazilian hospitals: characterization of virulence genes by whole genome sequencing

被引：5

作者：

Rizek, Camila Fonseca ^{[1
]}

Martins, Roberta Cristina ^{[1
]}

Girao, Evelyne Santana ^{[2
,3
,4
]}

Tavares, Bruno de Melo ^{[3
]}

Dos Santos, Sania Alves ^{[1
]}

Gamarra, Gessica Lorena ^{[1
]}

Perdigao Neto, Lauro Vieira ^{[1
,3
]}

Diogo, Constancia ^{[1
]}

Orsi, Tatiana D' Annibale ^{[1
]}

Boszczowski, Icaro ^{[3
,5
]}

Piastrelli, Filipe ^{[5
]}

Costa, Cecilia Leite ^{[6
]}

Costa, Daniely Viana ^{[2
]}

Maciel, Geovania ^{[2
]}

Romao, Janete ^{[4
]}

de Castro Brito, Gerly Anne ^{[6
]}

Costa, Silvia Figueiredo ^{[1
,3
]}

机构：

[1] Univ Sao Paulo, Lab Invest Med 49, Inst Med Trop, Fac Med, Av Dr Eneas De Carvalho Aguiar 470, Sao Paulo, Brazil

[2] Univ Fed Ceara, Hosp Univ Walter Cantidio, R Pastor Samuel Munguba 1290, Fortaleza, Ceara, Brazil

[3] Univ Sao Paulo, Fac Med, Hosp Clin, Av Dr Eneas De Carvalho Aguiar,255, Sao Paulo, Brazil

[4] Hosp Sao Jose Doencas Infecciosas, R Nestor Barbosa 315, Fortaleza, Ceara, Brazil

[5] Hosp Alemao Oswaldo Cruz, R Treze De Maio 1815, Sao Paulo, Brazil

[6] Univ Fed Ceara, Dept Morfol, Fortaleza, Ceara, Brazil

来源：

MICROBES AND INFECTION | 2022年 / 24卷 / 05期

关键词：

Clostridioides dif ficile; Virulence; Toxins;

D O I：

10.1016/j.micinf.2022.104953

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Clostridioides difficile (CD) is the most frequent cause of healthcare related diarrhea and its severity has increased in the last decade by the spread of hypervirulent strains. Most important CD virulence factor is toxin production; however, not only toxins are responsible for Clostridioides virulence. We sequenced 38 strains and analyzed the presence and integrity of 24 virulence (including toxin) genes. We identified 28 toxigenic strains, six also presented the cdt genes. Only six strains didn't present all others genes searched. All absent genes were adhesion related. Understand others CD virulence factors can lead to a best understanding on this matter. (C) 2022 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

引用

页数：5

共 16 条

[1] A Review of Clostridioides difficile Infection and Antibiotic-Associated Diarrhea
Abad, Cybele Lara R.
Safdar, Nasia
[J]. GASTROENTEROLOGY CLINICS OF NORTH AMERICA, 2021, 50 (02) : 323 - 340
[2] Clostridium difficile virulence factors: Insights into an anaerobic spore-forming pathogen
Awad, Milena M.
Johanesen, Priscilla A.
Carter, Glen P.
Rose, Edward
Lyras, Dena
[J]. GUT MICROBES, 2014, 5 (05) : 579 - 593
[3] The structure of the S-layer of Clostridium difficile
Bradshaw, William J.
Roberts, April K.
Shone, Clifford C.
Acharya, K. Ravi
[J]. JOURNAL OF CELL COMMUNICATION AND SIGNALING, 2018, 12 (01) : 319 - 331
[4] Molecular epidemiology and antimicrobial susceptibility of Clostridium difficile isolated from hospitals during a 4-year period in China
Chen, Yun-Bo
Gu, Si-Lan
Shen, Ping
Lv, Tao
Fang, Yun-Hui
Tang, Ling-Ling
Li, Lan-Juan
[J]. JOURNAL OF MEDICAL MICROBIOLOGY, 2018, 67 (01) : 52 - 59
[5] Predictive factors, outcomes, and molecular epidemiology of Clostridioides difficile diarrhea in Brazilian hospitals
Girao, Evelyne Santana
de Melo Tavares, Bruno
dos Santos, Sania Alves
Gamarra, Gessica Lorena
Rizek, Camila
Martins, Roberta Cristina
Neto, Lauro Vieira Perdigao
Diogo, Constancia
D' Annibale Orsi, Tatiana
Morales, Hugo Manuel Paz
da Silva Nogueira, Keite
Maestri, Adriane Ceshin
Boszczowski, Icaro
Piastrelli, Filipe
Costa, Cecilia Leite
Costa, Daniely Viana
Maciel, Geovania
Romao, Janete
Guimaraes, Thais
de Castro Brito, Gerly Anne
Costa, Silvia Figueiredo
[J]. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2021, 40 (09) : 1821 - 1832
[6] Genomic diversity of Clostridium difficile strains
Janezic, Sandra
Rupnik, Maja
[J]. RESEARCH IN MICROBIOLOGY, 2015, 166 (04) : 353 - 360
[7] Virulence factors of Clostridium difficile and their role during infection
Janoir, Claire
[J]. ANAEROBE, 2016, 37 : 13 - 24
[8] Mancini Alessio, 2018, Infez Med, V26, P204
[9] Clostridium difficile toxin expression is inhibited by the novel regulator TcdC
Matamouros, Susana
England, Patrick
Dupuy, Bruno
[J]. MOLECULAR MICROBIOLOGY, 2007, 64 (05) : 1274 - 1288
[10] Diverting Loop Ileostomy and Colonic Lavage An Alternative to Total Abdominal Colectomy for the Treatment of Severe, Complicated Clostridium difficile Associated Disease
Neal, Matthew D.
Alverdy, John C.
Hall, Daniel E.
Simmons, Richard L.
Zuckerbraun, Brian S.
[J]. ANNALS OF SURGERY, 2011, 254 (03) : 423 - 429

← 1 2 →