Vitamin C treatment promotes mesenchymal stem cell sheet formation and tissue regeneration by elevating telomerase activity

被引：250

作者：

Wei, Fulan ^{[1
]}

Qu, Cunye ^{[2
]}

Song, Tieli ^{[1
]}

Ding, Gang ^{[1
]}

Fan, Zhipeng ^{[1
]}

Liu, Dayong ^{[1
]}

Liu, Yi ^{[1
]}

Zhang, Chunmei ^{[1
]}

Shi, Songtao ^{[2
]}

Wang, Songlin ^{[1
,3
]}

机构：

[1] Capital Med Univ Sch Stomatol, Mol Lab Gene Therapy & Tooth Regenerat, Beijing Key Lab Tooth Regenerat & Funct Reconstru, Beijing 100050, Peoples R China

[2] Univ So Calif, Ctr Craniofacial Mol Biol, Herman Ostrow Sch Dent, Los Angeles, CA 90033 USA

[3] Capital Med Univ Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100050, Peoples R China

来源：

JOURNAL OF CELLULAR PHYSIOLOGY | 2012年 / 227卷 / 09期

基金：

美国国家卫生研究院;

关键词：

ASCORBIC-ACID; IN-VITRO; DIFFERENTIATION; PROLIFERATION; PERIODONTITIS; SYSTEM;

D O I：

10.1002/jcp.24012

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Cell sheet engineering has been developed as an alternative approach to improve mesenchymal stem cell-mediated tissue regeneration. In this study, we found that vitamin C (Vc) was capable of inducing telomerase activity in periodontal ligament stem cells (PDLSCs), leading to the up-regulated expression of extracellular matrix type I collagen, fibronectin, and integrin beta 1, stem cell markers Oct4, Sox2, and Nanog as well as osteogenic markers RUNX2, ALP, OCN. Under Vc treatment, PDLSCs can form cell sheet structures because of increased cell matrix production. Interestingly, PDLSC sheets demonstrated a significant improvement in tissue regeneration compared with untreated control dissociated PDLSCs and offered an effective treatment for periodontal defects in a swine model. In addition, bone marrow mesenchymal stem cell sheets and umbilical cord mesenchymal stem cell sheets were also well constructed using this method. The development of Vc-mediated mesenchymal stem cell sheets may provide an easy and practical approach for cell-based tissue regeneration. J. Cell. Physiol. 227: 32163224, 2012. (C)2011 Wiley Periodicals, Inc.

引用

页码：3216 / 3224

页数：9

共 40 条

[1] Extension of life-span by introduction of telomerase into normal human cells [J].