Alteration of N6-methyladenosine epitranscriptome profile in unilateral ureteral obstructive nephropathy

被引：29

作者：

Li, Xueyan ^{[1
]}

Fan, Xu ^{[1
]}

Yin, Xiaoming ^{[1
]}

Liu, Huajian ^{[1
]}

Yang, Yi ^{[1
]}

机构：

[1] China Med Univ, Shengjing Hosp, Dept Pediat Urol, Shenyang 110004, Liaoning, Peoples R China

来源：

EPIGENOMICS | 2020年 / 12卷 / 14期

基金：

中国国家自然科学基金;

关键词：

mRNA; N-6-methyladenosine (m(6)A); obstructive nephropathy; renal interstitial fibrosis; unilateral ureteral obstruction (UUO); RENAL FIBROSIS; MESENCHYMAL TRANSITION; KIDNEY-DISEASE; RNA; TRANSLATION; SMAD7; METHYLATION; EXPRESSION; CELLS; MODEL;

D O I：

10.2217/epi-2020-0126

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Aim: To reveal the alterations of N-6-methyladenosine (m(6)A) epitranscriptome profile in kidney after unilateral ureteral obstruction in mice. Materials & methods: Total renal m(6)A and expressions of methyltransferases and demethylases were detected by colorimetric quantification method, real-time PCR and western blot, respectively. Methylated RNA immunoprecipitation sequencing was performed to map epitranscriptome-wide m(6)A profile. Results: Total m(6)A levels were time-dependent decreased within 1 week, with the lowest level detected at day 7. A total of 823 differentially methylated transcripts in 507 genes were identified. Specifically, demethylated mRNAs selectively acted on multiple pathways, including TGF-beta and WNT. Conclusion: m(6)A modification has a functional importance in renal interstitial fibrosis during obstructive nephropathy and might be a promising therapeutic target.

引用

页码：1157 / 1173

页数：17

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