Electrochemical stimulation of microbial cis-dichloroethene (cis-DCE) oxidation by an ethene-assimilating culture

被引：38

作者：

Aulenta, Federico ^{[1
]}

Verdini, Roberta ^{[2
]}

Zeppilli, Marco ^{[2
]}

Zanaroli, Giulio ^{[3
]}

Fava, Fabio ^{[3
]}

Rossetti, Simona ^{[1
]}

Majone, Mauro ^{[2
]}

机构：

[1] Natl Res Council IRSA CNR, Water Res Inst, I-00015 Monterotondo, RM, Italy

[2] Univ Roma La Sapienza, Dept Chem, I-00185 Rome, Italy

[3] Univ Bologna, Dept Civil Chem Environm & Mat Engn, I-40131 Bologna, Italy

来源：

NEW BIOTECHNOLOGY | 2013年 / 30卷 / 06期

关键词：

ELECTRON-ACCEPTING CONDITIONS; VINYL-CHLORIDE; CHLORINATED SOLVENTS; REDUCTIVE DECHLORINATION; ANAEROBIC BIOREMEDIATION; AEROBIC BIODEGRADATION; CONTAMINATED SITES; SIDE REACTIONS; HUMIC ACIDS; BACTERIA;

D O I：

10.1016/j.nbt.2013.04.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This study investigated the feasibility of using a polarized graphite electrode as direct or indirect (via electrolytic oxygen generation) electron acceptor to stimulate the microbial oxidation of cis-dichloroethene (cis-DCE) in contaminated groundwater. A microbial culture was enriched in the anode chamber of a bioelectrochemical cell using a mixture of cis-DCE and ethene as substrates. The bioelectrochemical cell was operated by controlling the anode potential at +1.0 V or +1.5 V versus the standard hydrogen electrode (SHE). Enhanced cometabolic removal of cis-DCE, with ethene serving as the growth substrate, was observed in batch tests with the anode polarized at +1.5 V versus SHE. At this potential, (chloro) ethenes removal was probably sustained by molecular oxygen generated at the anode from water oxidation. Conversely, negligible anaerobic degradation was observed at +1.0 V versus SHE (a potential which does not allow oxygen generation), hence suggesting that molecular oxygen is needed to initiate (chloro) ethene degradation. PCR-DGGE analysis of the microbial culture followed by band sequencing and phylogenetic analysis evidenced the selective enrichment of a Bacillus species, providing a strong indication that this microorganism was responsible for cis-DCE and ethene degradation.

引用

页码：749 / 755

页数：7