Exciplexes and conical intersections lead to fluorescence quenching in π-stacked dimers of 2-aminopurine with natural purine nucleobases

被引：32

作者：

Liang, JingXin ^{[1
]}

Nguyen, Quynh L. ^{[1
]}

Matsika, Spiridoula ^{[1
]}

机构：

[1] Temple Univ, Dept Chem, Philadelphia, PA 19122 USA

来源：

PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES | 2013年 / 12卷 / 08期

基金：

美国国家科学基金会;

关键词：

EXCITED-STATE DYNAMICS; ULTRAFAST TRANSIENT-ABSORPTION; TIME-RESOLVED FLUORESCENCE; CHARGE-TRANSFER; ELECTRONIC STATES; STEADY-STATE; AB-INITIO; DNA-BASE; EXCIMER FORMATION; ADENINE;

D O I：

10.1039/c3pp25449f

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fluorescent analogues of the natural DNA bases are useful in the study of nucleic acids' structure and dynamics. 2-Aminopurine (2AP) is a widely used analogue with environmentally sensitive fluorescence behavior. The quantum yield of 2AP has been found to be significantly decreased when engaged in pi-stacking interactions with the native bases. We present a theoretical study on fluorescence quenching mechanisms in dimers of 2AP pi-stacked with adenine or guanine as in natural DNA. Relaxation pathways on the potential energy surfaces of the first excited states have been computed and reveal the importance of exciplexes and conical intersections in the fluorescence quenching process.

引用

页码：1387 / 1400

页数：14

共 94 条

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