Polynucleobacter necessarius, a model for genome reduction in both free-living and symbiotic bacteria

被引：58

作者：

Boscaro, Vittorio ^{[1
]}

Felletti, Michele ^{[1
]}

Vannini, Claudia ^{[1
]}

Ackerman, Matthew S. ^{[2
]}

Chain, Patrick S. G. ^{[3
]}

Malfatti, Stephanie ^{[4
]}

Vergez, Lisa M. ^{[5
]}

Shin, Maria ^{[6
]}

Doak, Thomas G. ^{[2
]}

Lynch, Michael ^{[2
]}

Petroni, Giulio ^{[1
]}

机构：

[1] Univ Pisa, Dept Biol, I-56126 Pisa, Italy

[2] Indiana Univ, Dept Biol, Bloomington, IN 47401 USA

[3] Los Alamos Natl Lab, Los Alamos, NM 87545 USA

[4] Joint Genome Inst, Walnut Creek, CA 94598 USA

[5] Lawrence Livermore Natl Lab, Livermore, CA 94550 USA

[6] Eureka Genom, Hercules, CA 94547 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2013年 / 110卷 / 46期

基金：

美国国家科学基金会;

关键词：

symbiosis; nonsynonymous mutation rates; Burkholderiales; protozoa; genome streamlining; DNA-POLYMERASES; WATER HABITATS; EVOLUTION; SEQUENCE; REVEALS; ENDOSYMBIONTS; ASYMBIOTICUS; ALIGNMENT; BUCHNERA;

D O I：

10.1073/pnas.1316687110

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We present the complete genomic sequence of the essential symbiont Polynucleobacter necessarius (Betaproteobacteria), which is a valuable case study for several reasons. First, it is hosted by a ciliated protist, Euplotes; bacterial symbionts of ciliates are still poorly known because of a lack of extensive molecular data. Second, the single species P. necessarius contains both symbiotic and free-living strains, allowing for a comparison between closely related organisms with different ecologies. Third, free-living P. necessarius strains are exceptional by themselves because of their small genome size, reduced metabolic flexibility, and high worldwide abundance in freshwater systems. We provide a comparative analysis of P. necessarius metabolism and explore the peculiar features of a genome reduction that occurred on an already streamlined genome. We compare this unusual system with current hypotheses for genome erosion in symbionts and free-living bacteria, propose modifications to the presently accepted model, and discuss the potential consequences of translesion DNA polymerase loss.

引用

页码：18590 / 18595

页数：6

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[1] BASIC LOCAL ALIGNMENT SEARCH TOOL [J].