FK506 induces chondrogenic differentiation of clonal mouse embryonic carcinoma cells, ATDC5

被引：28

作者：

Nishigaki, F ^{[1
]}

Sakuma, S ^{[1
]}

Ogawa, T ^{[1
]}

Miyata, S ^{[1
]}

Ohkubo, T ^{[1
]}

Goto, T ^{[1
]}

机构：

[1] Fujisawa Pharmaceut Co Ltd, Med Biol Res Labs, Osaka 5328514, Japan

来源：

EUROPEAN JOURNAL OF PHARMACOLOGY | 2002年 / 437卷 / 03期

关键词：

FK506; cyclosporin A; ATDC5; cell; chondrogenic differentiation; calcineurin; cartilage;

D O I：

10.1016/S0014-2999(02)01269-4

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

FK506 (Tacrolimus) and cyclosporin A exert their immunosuppressive effects via a common mechanism, calcineurin inhibition, after binding to intracellular proteins termed immunophilins: FK506-binding protein (FKBP) and cyclophilin. In this study, FK506 was found to induce chondrogenic differentiation of ATDC5 cells (clonal mouse embryonal carcinoma cells) in a concentration-dependent manner (0.1-1000 ng/ml). Immunohisocheical staining showed that ATDC5 cells induced to differentiate by FK506 produced proteoglycan and type 11 collagen, main components of the extracellular matrix of cartilage. Rapamycin, an immunosuppressant that binds to FKBP, antagonized the effect of FK506. Cyclosporin A did not induce chondrogenesis at concentrations up to 1000 ng/ml. Taken together, these results suggest that FK506 induces chondrogenic differentiation of ATDC5 cells via a calcineurin-independent mechanism, after binding to FKBP. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：123 / 128

页数：6

共 16 条

[1] Osteogenic phenotype expression of allogeneic rat marrow cells in porous hydroxyapatite ceramics
Akahane, M
Ohgushi, H
Yoshikawa, T
Sempuku, T
Tamai, S
Tabata, S
Dohi, Y
[J]. JOURNAL OF BONE AND MINERAL RESEARCH, 1999, 14 (04) : 561 - 568
[2] Akiyama H, 1996, J BONE MINER RES, V11, P22
[3] Cloning of a mouse smoothened cDNA and expression patterns of hedgehog signalling molecules during chondrogenesis and cartilage differentiation in clonal mouse EC cells, ATDC5
Akiyama, H
Shigeno, C
Hiraki, Y
Shukunami, C
Kohno, H
Akagi, M
Konishi, J
Nakamura, T
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1997, 235 (01) : 142 - 147
[4] A CHONDROGENIC CELL-LINE DERIVED FROM A DIFFERENTIATING CULTURE OF AT805 TERATOCARCINOMA CELLS
ATSUMI, T
MIWA, Y
KIMATA, K
IKAWA, Y
[J]. CELL DIFFERENTIATION AND DEVELOPMENT, 1990, 30 (02): : 109 - 116
[5] 2 DISTINCT SIGNAL TRANSMISSION PATHWAYS IN LYMPHOCYTES-T ARE INHIBITED BY COMPLEXES FORMED BETWEEN AN IMMUNOPHILIN AND EITHER FK506 OR RAPAMYCIN
BIERER, BE
MATTILA, PS
STANDAERT, RF
HERZENBERG, LA
BURAKOFF, SJ
CRABTREE, G
SCHREIBER, SL
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (23) : 9231 - 9235
[6] Furst DE, 1999, ARTHRITIS RHEUM, V42, pS271
[7] Lesser reduction in bone mineral density by the immunosuppressant, FK506, compared with cyclosporine in rats
Inoue, T
Kawamura, I
Matsuo, I
Aketa, M
Mabuchi, M
Seki, J
Goto, T
[J]. TRANSPLANTATION, 2000, 70 (05) : 774 - 779
[8] Bone morphogenetic protein-6 and parathyroid hormone-related protein coordinately regulate the hypertrophic conversion in mouse clonal chondrogenic EC cells, ATDC5
Ito, H
Akiyama, H
Shigeno, C
Nakamura, T
[J]. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 1999, 1451 (2-3): : 263 - 270
[9] Osteoblastic differentiation is enhanced by rapamycin in rat osteoblast-like osteosarcoma (ROS 17/2.8) cells
Ogawa, T
Tokuda, M
Tomizawa, K
Matsui, H
Itano, T
Konishi, R
Nagahata, S
Hatasa, O
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1998, 249 (01) : 226 - 230
[10] The nuclear factor of activated T cells (NFAT) transcription factor NFATp (NFATc2) is a repressor of chondrogenesis
Ranger, AM
Gerstenfeld, LC
Wang, JX
Kon, T
Bae, HS
Gravallese, EM
Glimcher, MJ
Glimcher, LH
[J]. JOURNAL OF EXPERIMENTAL MEDICINE, 2000, 191 (01) : 9 - 21

← 1 2 →