PKC-dependent phosphorylation of the p97 repressor regulates the transcription of aldolase A L-type promoter

被引：8

作者：

Costanzo, P ^{[1
]}

Lupo, A ^{[1
]}

Medugno, L ^{[1
]}

D'Agostino, P ^{[1
]}

Zevino, C ^{[1
]}

Izzo, P ^{[1
]}

机构：

[1] Univ Naples Federico II, Fac Med & Chirurg, Dipartimento Biochim & Biotecnol Med, I-80131 Naples, Italy

来源：

FEBS LETTERS | 1999年 / 454卷 / 1-2期

关键词：

negative cis element; protein kinase C; phosphorylation; cell cycle; transcriptional regulation;

D O I：

10.1016/S0014-5793(99)00775-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Expression of mouse aldolase A L-type mRNA is negatively modulated by a cis element (AldA-NRE), located within the aldolase A distal promoter (pL), AldA-NRE interacts with a 97-kDa repressor protein (p97), which binds DNA in a cell cycle-dependent manner. We demonstrate that the binding between AldA-NRE and p97 decreases during differentiation of human Caco-2 cells and is inversely correlated with L-type mRNA expression. Phosphorylation of the p97 repressor weakened its DNA binding activity in differentiated Caco-2 cells, while dephosphorylation enhanced the binding in proliferating cells. Stimulation of protein kinase C (PKC) in vivo decreased the binding of p97 to AldA-NRE and stimulated transcription, while inhibition of PKC stimulated p97 binding and downregulated transcription. These findings suggest that PKC is a mediator of the binding and silencing function of the p97/AldA-NRE repressor complex. (C) 1999 Federation of European Biochemical Societies.

引用

页码：61 / 66

页数：6

共 25 条

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