High-speed multi-exposure laser speckle contrast imaging with a single-photon counting camera

被引：48

作者：

Dragojevic, Tanja ^{[1
]}

Bronzi, Danilo ^{[2
]}

Varma, Hari M. ^{[1
]}

Valdes, Claudia P. ^{[1
]}

Castellvi, Clara ^{[3
]}

Villa, Federica ^{[2
]}

Tosi, Alberto ^{[2
]}

Justicia, Carles ^{[3
]}

Zappa, Franco ^{[2
]}

Durduran, Turgut ^{[1
]}

机构：

[1] ICFO Inst Ciencies Foton, Castelldefels 08860, Barcelona, Spain

[2] Politecn Milan, Dipartimento Elettron Informat & Bioingn, I-20133 Milan, Italy

[3] CSIC, Spanish Res Council, Inst Biomed Res IIBB, Dept Brain Ischemia & Neurodegenerat,Inst Invest, Barcelona, Spain

来源：

BIOMEDICAL OPTICS EXPRESS | 2015年 / 6卷 / 08期

关键词：

CEREBRAL-BLOOD-FLOW; FLOWMETRY; TISSUE; SPECTROSCOPY;

D O I：

10.1364/BOE.6.002865

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Laser speckle contrast imaging (LSCI) has emerged as a valuable tool for cerebral blood flow (CBF) imaging. We present a multi-exposure laser speckle imaging (MESI) method which uses a high-frame rate acquisition with a negligible inter-frame dead time to mimic multiple exposures in a single-shot acquisition series. Our approach takes advantage of the noise-free readout and high-sensitivity of a complementary metal-oxide- semiconductor (CMOS) single-photon avalanche diode (SPAD) array to provide real-time speckle contrast measurement with high temporal resolution and accuracy. To demonstrate its feasibility, we provide comparisons between in vivo measurements with both the standard and the new approach performed on a mouse brain, in identical conditions. (C) 2015 Optical Society of America

引用

页码：2865 / 2876

页数：12

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