Lipase-catalysed transesterification of high oleic sunflower oil

被引：145
作者：
Dossat, V [1 ]
Combes, D [1 ]
Marty, A [1 ]
机构：
[1] INRA, CNRS,Dept Genie Biochim & Alimentaire, UMR 5504,UMR 792, Ctr Bioingn Gilbert Durand,INSA, F-31077 Toulouse 4, France
来源：
ENZYME AND MICROBIAL TECHNOLOGY | 2002年 / 30卷 / 01期
关键词：
lipase; organic solvents; transesterification; alcoholysis; kinetic; thermodynamic; solvent free media;
D O I：
10.1016/S0141-0229(01)00453-7
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The study of enzymatic transesterification of high oleic sunflower oil with butanol by immobilised Lipozyme(R) was realised in n-hexane and in a solvent-free system. A simplified model, based on the Ping Pong Bi Bi with alcohol competitive inhibition mechanism, was proposed to describe transesterification kinetics, The solvent-free reaction medium was characterised by higher initial velocities and a 6-times volumic productivity but at the thermodynamic equilibrium only about 60% of the potential oleic acid is converted into ester, against 95% in n-hexane. The resulting product is a mixture of ester, monoglyceride, diglyceride and triglyceride with lubricant and surfactant properties and its composition depends on the initial substrate ratio. (C) 2002 Elsevier Science Inc. All rights reserved.
引用
收藏
页码：90 / 94
页数：5
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