Trans-unsaturated fatty acid activates NLRP3 inflammasome in macrophages and exacerbates intestinal inflammation in mice

被引：8

作者：

Higashimura, Yasuki ^{[1
,2
]}

Tanaka, Yumiko ^{[1
]}

Takagi, Tomohisa ^{[2
]}

Uchiyama, Kazuhiko ^{[2
]}

Mizushima, Katsura ^{[2
]}

Niki, Etsuo ^{[3
]}

Naito, Yuji ^{[2
]}

机构：

[1] Ishikawa Prefectural Univ, Dept Food Sci, Nonoichi, Ishikawa 9218836, Japan

[2] Kyoto Prefectural Univ Med, Grad Sch Med Sci, Mol Gastroenterol & Hepatol, Kyoto 6028566, Japan

[3] Univ Tokyo, Res Ctr Adv Sci & Technol, Meguro Ku, Tokyo 1530041, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2020年 / 529卷 / 02期

基金：

日本学术振兴会;

关键词：

Colon; Elaidate; Endoplasmic reticulum stress; Interleukin-1; beta; Reactive oxygen species; OXIDATIVE STRESS; INDUCTION; COLITIS; ADULTS;

D O I：

10.1016/j.bbrc.2020.06.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Higher consumption of trans fatty acid (TFA) is a risk factor for several inflammatory diseases including inflammatory bowel disease (IBD). However, the detailed mechanisms by which TFA intake affects IBD pathology remain unclear. We demonstrate here that elaidate, a trans-isomer of oleate, enhances interleukin (IL)-1 beta production through the activation of NLRP3 inflammasome in mouse bone marrow-derived macrophages (BMDMs). Oleate has no effect on IL-1 beta production. Elaidate also induces oxidative stress and activates endoplasmic reticulum stress in BMDMs. The elaidate-induced IL-1 beta production is suppressed by co-treatments with antioxidants and a chemical chaperone. Furthermore, we analyze the effects of elaidate administration on intestinal inflammation using 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis model in mice. Increased colonic damage and myeloperoxidase activity after TNBS treatment are elevated by elaidate administration. Also, TNBS treatment induces IL-1 beta production in colonic mucosa; elaidate administration enhances the induction. We believe that these data reveal some mechanisms by which the TFA intake is associated with increased risk for IBD. (C) 2020 Elsevier Inc. All rights reserved.

引用

页码：243 / 250

页数：8

共 23 条

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