High-Throughput Microfluidic Device for Rare Cell Isolation

被引：5

作者：

Yang, Daniel ^{[1
]}

Leong, Serena ^{[2
]}

Lei, Andy ^{[2
]}

Sohn, Lydia L. ^{[1
]}

机构：

[1] Univ Calif Berkeley, Dept Mech Engn, Berkeley, CA 94720 USA

[2] Univ Calif Berkeley, Dept Bioengn, Berkeley, CA 94720 USA

来源：

BIO-MEMS AND MEDICAL MICRODEVICES II | 2015年 / 9518卷

关键词：

CIRCULATING TUMOR-CELLS; WHOLE-BLOOD; SEPARATION;

D O I：

10.1117/12.2178613

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Enumerating and analyzing circulating tumor cells (CTCs)-cells that have been shed from primary solid tumors-can potentially be used to determine patient prognosis and track the progression of disease. There is a great challenge to create an effective platform that can isolate these cells, as they are extremely rare: only 1-10 CTCs are present in a 7.5mL of a cancer patient's peripheral blood. We have developed a novel microfluidic system that can isolate CTC populations label free. Our system consists of a multistage separator that employs inertial migration to sort cells based on size. We demonstrate the feasibility of our device by sorting colloids that are comparable in size to red blood cells (RBCs) and CTCs.

引用

页数：7

共 11 条

[1] Inertial microfluidic physics
Amini, Hamed
Lee, Wonhee
Di Carlo, Dino
[J]. LAB ON A CHIP, 2014, 14 (15) : 2739 - 2761
[2] Microfluidic: An innovative tool for efficient cell sorting
Autebert, Julien
Coudert, Benoit
Bidard, Francois-Clement
Pierga, Jean-Yves
Descroix, Stephanie
Malaquin, Laurent
Viovy, Jean-Louis
[J]. METHODS, 2012, 57 (03) : 297 - 307
[3] Equilibrium separation and filtration of particles using differential inertial focusing
Di Carlo, Dino
Edd, Jon F.
Irimia, Daniel
Tompkins, Ronald G.
Toner, Mehmet
[J]. ANALYTICAL CHEMISTRY, 2008, 80 (06) : 2204 - 2211
[4] Label-free cell separation and sorting in microfluidic systems
Gossett, Daniel R.
Weaver, Westbrook M.
Mach, Albert J.
Hur, Soojung Claire
Tse, Henry Tat Kwong
Lee, Wonhee
Amini, Hamed
Di Carlo, Dino
[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2010, 397 (08) : 3249 - 3267
[5] Detecting Circulating Tumor Cells: Current Challenges and New Trends
Hong, Bin
Zu, Youli
[J]. THERANOSTICS, 2013, 3 (06): : 377 - 394
[6] Label-Free Cancer Cell Separation from Human Whole Blood Using Inertial Microfluidics at Low Shear Stress
Lee, Myung Gwon
Shin, Joong Ho
Bae, Chae Yun
Choi, Sungyoung
Park, Je-Kyun
[J]. ANALYTICAL CHEMISTRY, 2013, 85 (13) : 6213 - 6218
[7] Continuous flow separations in microfluidic devices
Pamme, Nicole
[J]. LAB ON A CHIP, 2007, 7 (12) : 1644 - 1659
[8] Highly Efficient Assay of Circulating Tumor Cells by Selective Sedimentation with a Density Gradient Medium and Microfiltration from Whole Blood
Park, Jong-Myeon
Lee, June-Young
Lee, Jeong-Gun
Jeong, Hyoyoung
Oh, Jin-Mi
Kim, Yeon Jeong
Park, Donghyun
Kim, Minseok S.
Lee, Hun Joo
Oh, Jin Ho
Lee, Soo Suk
Lee, Won-Yong
Huh, Nam
[J]. ANALYTICAL CHEMISTRY, 2012, 84 (17) : 7400 - 7407
[9] Optical quantification of cellular mass, volume, and density of circulating tumor cells identified in an ovarian cancer patient
Phillips, Kevin G.
Velasco, Carmen Ruiz
Lie, Julia
Kolatkar, Anand
Luttgen, Madelyn
Bethel, Kelly
Duggan, Bridgette
Kuhn, Peter
McCarty, Owen J. T.
[J]. FRONTIERS IN ONCOLOGY, 2012, 2 : 1 - 8
[10] Isolation by size of epithelial tumor cells -: A new method for the immunomorphological and molecular characterization of circulating tumor cells
Vona, G
Sabile, A
Louha, M
Sitruk, V
Romana, S
Schütze, K
Capron, F
Franco, D
Pazzagli, M
Vekemans, M
Lacour, B
Bréchot, C
Paterlini-Bréchot, P
[J]. AMERICAN JOURNAL OF PATHOLOGY, 2000, 156 (01) : 57 - 63

← 1 2 →