Ste20-related protein kinase LOSK (SLK) controls microtubule radial array in interphase

被引:27
作者
Burakov, Anton V. [1 ,2 ]
Zhapparova, Olga N. [1 ,3 ]
Kovalenko, Olga V. [2 ]
Zinovkina, Liudmila A. [2 ]
Potekhina, Ekaterina S. [2 ]
Shanina, Nina A. [3 ]
Weiss, Dieter G. [4 ]
Kuznetsov, Sergei A. [4 ]
Nadezhdina, Elena S. [1 ]
机构
[1] Russian Acad Sci, Inst Prot Res, Pushchino 142290, Moscow Region, Russia
[2] Moscow MV Lomonosov State Univ, AN Belozersky Inst Physicochem Biol, Moscow 119899, Russia
[3] Moscow MV Lomonosov State Univ, Fac Biol, Moscow 119899, Russia
[4] Univ Rostock, Inst Biol Sci Cell Biol & Biosyst Technol, D-18059 Rostock, Germany
基金
俄罗斯基础研究基金会;
关键词
D O I
10.1091/mbc.E06-12-1156
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Interphase microtubules are organized into a radial array with centrosome in the center. This organization is a subject of cellular regulation that can be driven by protein phosphorylation. Only few protein kinases that regulate microtubule array in interphase cells have been described. Ste20-like protein kinase LOSK (SLK) was identified as a microtubule and centrosome-associated protein. In this study we have shown that the inhibition of LOSK activity by dominant-negative mutant K63R-Delta T or by LOSK depletion with RNAi leads to unfocused microtubule arrangement. Microtubule disorganization is prominent in Vero, CV-1, and CHO-K1 cells but less distinct in HeLa cells. The effect is a result neither of microtubule stabilization nor of centrosome disruption. In cells with suppressed LOSK activity centrosomes are unable to anchor or to cap microtubules, though they keep nucleating microtubules. These centrosomes are depleted of dynactin. Vero cells overexpressing K63R-Delta T have normal dynactin "comets" at microtubule ends and unaltered morphology of Golgi complex but are unable to polarize it at the wound edge. We conclude that protein kinase LOSK is required for radial microtubule organization and for the proper localization of Golgi complex in various cell types.
引用
收藏
页码:1952 / 1961
页数:10
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