Simultaneous analysis of prostanoids using liquid chromatography/high-field asymmetric waveform ion mobility spectrometry/tandem mass spectrometry

被引：39
|
作者：
Kapron, James
Wu, Jin
Mauriala, Timo
Clark, Patsy
Purves, Randy W.
Bateman, Kevin P.
机构：
[1] Merck Frosst Ctr Therapeut Res, Kirkland, PQ H9H 3L1, Canada
[2] Ionalytics, Ottawa, ON K1A 0R6, Canada
来源：
RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2006年 / 20卷 / 10期
关键词：
D O I：
10.1002/rcm.2505
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A liquid chromatography/high-field asymmetric waveform ion mobility spectrometry/tandem mass spectrometry (LC-FAIMS-MS/MS) semi-quantitative method was developed for the simultaneous determination of prostaglandin (PG) E-2, PGD(2), PGF(2 alpha), 6-keto-PGF(1 alpha), and thromboxane (TX) B-2. Diluted samples containing these prostanoids and their tetra-deuterium-substituted internal standards were analyzed by LC followed by either selected reaction monitoring (LC-SRM) or FAIMS and selected reaction monitoring (LC-FRM). FAIMS reduced background noise, separated the isobaric ions PGE(2) and PGD(2), and separated dynamically interchanging TXB2 anomers. This is the first report of the separation of interconverting anomers by FAIMS. Generally, the LC-FRM chromatograms were more selective than the LC-SRM chromatograms. Its potential was demonstrated by analysis of prostanoids in guinea pig lumbar spinal cord homogenate. Copyright (c) 2006 John Wiley & Sons, Ltd.
引用
收藏
页码：1504 / 1510
页数：7
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