Overexpression, crystallization and preliminary X-ray crystallographic analysis of a putative xylose isomerase from Bacteroides thetaiotaomicron

被引：3

作者：

Cho, Jea-Won ^{[1
,2
]}

Han, Byeong-Gu ^{[2
]}

Park, Sang Youn ^{[3
]}

Kim, Seung Jun ^{[4
]}

Kim, Myoung-Dong ^{[1
]}

Lee, Byung Il ^{[2
]}

机构：

[1] Kangwon Natl Univ, Dept Food Sci & Biotechnol, Chunchon 200701, South Korea

[2] Natl Canc Ctr, Res Inst, Div Convergence Technol, Biomol Funct Res Branch, Goyang 410769, Gyeonggi, South Korea

[3] Soongsil Univ, Sch Syst Biomed Sci, Seoul 156743, South Korea

[4] Korea Res Inst Biosci & Biotechnol, Med Prote Res Ctr, Taejon 305333, South Korea

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2013年 / 69卷

基金：

新加坡国家研究基金会;

关键词：

GLUCOSE-ISOMERASE;

D O I：

10.1107/S1744309113023877

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Bacteroides thetaiotaomicron BT0793, a putative xylose isomerase, was overexpressed in Escherichia coli, purified and crystallized using polyethylene glycol monomethyl ether 550 as the precipitant. X-ray diffraction data were collected to 2.10 angstrom resolution at 100 Kusing synchrotron X-rays. The crystal was found to belong to space group P1, with unit-cell parameters a = 96.3, b = 101.7, c = 108.3 angstrom, alpha = 82.8, beta = 68.2, gamma = 83.0 degrees. The asymmetric unit contained eight subunits of xylose isomerase with a crystal volume per protein weight (V-M) of 2.38 angstrom(3) Da(-1) and a solvent content of 48.3%.

引用

页码：1127 / 1130

页数：4

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