Cytosolic glutamine synthetase is present in the phloem sap of rice (Oryza sativa L.)

被引：7
作者：
Tanaka, Tomohiro
Suzui, Nobuo
Hayashi, Hiroaki
Yamaya, Tomoyuki [2 ]
Yoneyama, Tadakatsu [1 ]
机构：
[1] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Appl Biol Chem, Bunkyo Ku, Tokyo 1138657, Japan
[2] Tohoku Univ, Dept Appl Biol Chem, Fac Agr, Sendai, Miyagi 9818555, Japan
来源：
SOIL SCIENCE AND PLANT NUTRITION | 2009年 / 55卷 / 01期
关键词：
glutamine synthetase; GS1; nitrogen; phloem sap; rice (Oryza sativa L; NATURAL SENESCENCE; SIEVE-TUBE; PLANTS; LEAVES; PROTEIN; NITROGEN; GROWTH; REMOBILIZATION; PLASMODESMATA; LOCALIZATION;
D O I：
10.1111/j.1747-0765.2008.00327.x
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
The presence of glutamine synthetase (GS) in the rice sieve tube was examined. Proteins in the rice phloem sap from leaf sheaths were separated by sodium dodecylsulfate-polyacrylamide gel electrophoresis, transferred to polyvinylidine difluoride membranes and immunoblotted with anti-GS1 antibody. A cross-reacting band, thought to be GS1, was detected in the phloem sap. Moreover, the phloem sap contained a significant amount of GS transferase activity. Previous studies have shown that the concentrations of substrates and cofactors in the rice phloem sap are sufficient for cytosolic GS reaction. These data suggest that physiologically active GS1 is present in rice phloem sap, which might convert glutamate to glutamine in vivo.
引用
收藏
页码：102 / 106
页数：5
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