Glaciecola lipolytica sp nov., isolated from seawater near Tianjin city, China

A Gram-negative, non-spore-forming, rod-shaped bacterium, designated strain E3(T), which was motile with a polar flagellum, was isolated from coastal surface seawater near Tianjin city, China. The taxonomy of strain E3(T) was studied by phenotypic, chemotaxonomic and phylogenetic methods. Strain E3(T) forms non-pigmented colonies with a butyrous-mucoid consistency on marine 2216 agar. It grows optimally at 30 degrees C, in the presence of 2.0 % (w/v) NaCl and at pH 7.6. Strain E3(T) is positive for catalase and oxidase and hydrolysis of lipid and reduces nitrate to N-2 gas. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain E3(T) belongs to the Gammaproteobacteria and is grouped in the genus Glaciecola, with highest sequence similarity to the type strains of Glaciecola chathamensis (94.6%), G. agarilytica (94.4%) and G. mesophila (94.4%). Strain E3(T) contained summed feature 3 (C-16:1 omega 7c and/or iso-C-15:0 2-OH, 34.5%), C-18:1 omega 7c (117.3%) and C-16:0 (17%) as the major fatty acids and phosphatidylethanolamine and phosphatidylglycerol as the main phospholipids. The DNA G+C content was 40.8 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain E3T is considered to represent a novel species of the genus Glaciecola, for which the name Glaciecola lipolytica sp. nov. is proposed. The type strain is E3(T) (=CGMCC 1.7001(T) =JCM 15139(T)).