The centriolar satellite protein SSX2IP promotes centrosome maturation

被引：54

作者：

Baerenz, Felix ^{[1
]}

Inoue, Daigo ^{[3
]}

Yokoyama, Hideki ^{[1
]}

Tegha-Dunghu, Justus ^{[1
]}

Freiss, Stephanie ^{[1
]}

Draeger, Stefanie ^{[1
]}

Mayilo, Dmytro ^{[1
]}

Cado, Ivana ^{[1
]}

Merker, Sabine ^{[1
]}

Klinger, Maren ^{[1
]}

Hoeckendorf, Burkhard ^{[3
]}

Pilz, Sahra ^{[1
]}

Hupfeld, Kerstin ^{[1
]}

Steinbeisser, Herbert ^{[2
]}

Lorenz, Holger ^{[1
]}

Ruppert, Thomas ^{[1
]}

Wittbrodt, Joachim ^{[3
]}

Gruss, Oliver J. ^{[1
]}

机构：

[1] Univ Heidelberg ZMBH, Zentrum Mol Biol, DKFZ ZMBH Alliance, D-69120 Heidelberg, Germany

[2] Heidelberg Univ, Inst Human Genet, D-69120 Heidelberg, Germany

[3] Heidelberg Univ, COS, D-69120 Heidelberg, Germany

来源：

JOURNAL OF CELL BIOLOGY | 2013年 / 202卷 / 01期

关键词：

HUMAN MITOTIC SPINDLE; XENOPUS EGG EXTRACTS; GAMMA-TUBULIN; TRANSLATIONAL CONTROL; DNA-DAMAGE; RAN; LOCALIZATION; MICROTUBULES; ORGANIZATION; STABILIZES;

D O I：

10.1083/jcb.201302122

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Meiotic maturation in vertebrate oocytes is an excellent model system for microtubule reorganization during M-phase spindle assembly. Here, we surveyed changes in the pattern of microtubule-interacting proteins upon Xenopus laevis oocyte maturation by quantitative proteomics. We identified the synovial sarcoma X breakpoint protein (SSX2IP) as a novel spindle protein. Using X. laevis egg extracts, we show that SSX2IP accumulated at spindle poles in a Dynein-dependent manner and interacted with the gamma.-tubulin ring complex (gamma-TuRC) and the centriolar satellite protein PCM-1. Immunodepletion of SSX2IP impeded gamma-TuRC loading onto centrosomes. This led to reduced microtubule nucleation and spindle assembly failure. In rapidly dividing blastomeres of medaka (Oryzias latipes) and in somatic cells, SSX2IP knockdown caused fragmentation of pericentriolar material and chromosome segregation errors. We characterize SSX2IP as a novel centrosome maturation and maintenance factor that is expressed at the onset of vertebrate development. It preserves centrosome integrity and faithful mitosis during the rapid cleavage division of blastomeres and in somatic cells.

引用

页码：81 / 95

页数：15

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