Characterization of a Bombyx mori nucleopolyhedrovirus with Bmvp80 disruption

被引：16
作者：
Tang, Xu-Dong [1 ]
Xu, Yi-Peng [1 ]
Yu, Lin-lin [1 ]
Lang, Guo-Jun [1 ]
Tian, Cai-Hong [1 ]
Zhao, Jin-Fang [1 ]
Zhang, Chuan-Xi [1 ]
机构：
[1] Zhejiang Univ, Inst Insect Sci, Hangzhou 310029, Zhejiang, Peoples R China
来源：
VIRUS RESEARCH | 2008年 / 138卷 / 1-2期
基金：
中国国家自然科学基金;
关键词：
Bombyx mori; Nucleopolyhedrovirus; Bmvp80; Ac104; Gene disruption; BV production;
D O I：
10.1016/j.virusres.2008.08.013
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A BmNPV Bacmid with the Bmvp80 gene disrupted was constructed using the ET-recombination system in Escherichia coli to investigate the role of Bmvp80 during the baculovirus life cycle. Disruption of Bmvp80 resulted in single cell infection phenotype, whereas a rescue BmBacmid restored budded virus titers to wild type levels; however, the homologous gene Ac104 (Acvp80) from AcMNPV could not complement the BmBacmid lacking a functional Bmvp80 gene. Electron microscopy of cells transfected with BmNPV lacking functional Bmvp80 revealed that the number of nucleocapsids was markedly lower. These results suggest that Bmvp80 is essential for normal budded virus production and nucleocapsid maturation, and is functionally divergent between baculovirus species. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码：81 / 88
页数：8
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