A large genome center's improvements to the Illumina sequencing system

被引：514

作者：

Quail, Michael A. ^{[1
]}

Kozarewa, Iwanka ^{[1
]}

Smith, Frances ^{[1
]}

Scally, Aylwyn ^{[1
]}

Stephens, Philip J. ^{[1
]}

Durbin, Richard ^{[1
]}

Swerdlow, Harold ^{[1
]}

Turner, Daniel J. ^{[1
]}

机构：

[1] Wellcome Trust Sanger Inst, Hinxton CB10 1SA, Cambs, England

来源：

NATURE METHODS | 2008年 / 5卷 / 12期

基金：

英国医学研究理事会; 英国惠康基金;

关键词：

D O I：

10.1038/nmeth.1270

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The Wellcome Trust Sanger Institute is one of the world's largest genome centers, and a substantial amount of our sequencing is performed with 'next-generation' massively parallel sequencing technologies: in June 2008 the quantity of purity-filtered sequence data generated by our Genome Analyzer (Illumina) platforms reached 1 terabase, and our average weekly Illumina production output is currently 64 gigabases. Here we describe a set of improvements we have made to the standard Illumina protocols to make the library preparation more reliable in a high-throughput environment, to reduce bias, tighten insert size distribution and reliably obtain high yields of data.

引用

页码：1005 / 1010

页数：6

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