Streptococcus mutans and Streptococcus sanguinis Expression of Competition-Related Genes, Under Sucrose

被引:14
作者
Lozano, Carla P. [1 ]
Diaz-Garrido, Natalia [2 ]
Kreth, Jens [3 ]
Giacaman, Rodrigo A. [2 ,4 ]
机构
[1] Univ Chile, Inst Res Dent Sci, Oral Biol & Biochem Lab, Fac Dent, Santiago, Chile
[2] Univ Talca, Dept Oral Rehabil, Cariol Unit, Talca, Chile
[3] Oregon Hlth & Sci Univ, Dept Restorat Dent, Portland, OR 97201 USA
[4] Univ Talca, Interdisciplinary Excellence Res Program Hlth Agi, Talca, Chile
关键词
Dental caries; Dual-species biofilm; Competition; Sucrose; Gene expression; DENTAL BIOFILM FORMATION; ORAL BACTERIA; CARIES; HEALTH; COLONIZATION; MATRIX;
D O I
10.1159/000490950
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Streptococcus mutans synthesizes 3 glucosyltransferases (Gtfs) associated with cariogenic biofilms, while commensal Streptococcus sanguinis produces only one; gtfP and hydrogen peroxide (H2O2) by SpxB. The aim was to test the hypothesis that under a sucrose-induced cariogenic challenge, the expression of competition-related genes is differentially regulated depending on whether S. sanguinis or S. mutans primarily colonize enamel. Dual-species biofilms of S. sanguinis and S. mutans were formed under different colonization sequences on enamel slabs and exposed to 10% sucrose for 5 min, 3x/day for 5 days. Biofilms were analyzed for the transcriptional response of competition-related genes encoding gtfB, gtfC, and gtfD for S. mutans and gtfP and spxB for S. sanguinis. In addition, acidogenicity (pH) and viable cells in each of the conditions were determined. For all the genes, a downregulation was observed during simultaneous colonization by both bacterial species. In contrast, gtfB was upregulated when S. sanguinis was the first colonizer (p < 0.05). Both gtfC and gtfD were upregulated during sequential inoculation with S. sanguinis as the first colonizer. An eleven-fold upregulation of gtfP was observed in biofilms with S. mutans as initial colonizer (p < 0.05), with a moderate increase in spxB expression. The lowest pH values and viable cells of S. sanguinis were observed when S. mutans first colonized the enamel slabs, compared to the other conditions (p < 0.05). Demanding sucrose-challenged oral environment requires increased expression of virulence traits to effectively compete and thrive in the dental biofilm, especially when the competitor has already colonized the ecological niche. (c) 2018 S. Karger AG, Basel
引用
收藏
页码:194 / 203
页数:10
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