Development of a germfree mouse model of Vibrio cholerae infection

被引:46
作者
Butterton, JR
Ryan, ET
Shahin, RA
Calderwood, SB
机构
[1] MASSACHUSETTS GEN HOSP,INFECT DIS UNIT,BOSTON,MA 02114
[2] US FDA,CTR BIOL EVALUAT & RES,LAB PERTUSSIS,BETHESDA,MD 20892
[3] HARVARD UNIV,SCH MED,DEPT MICROBIOL & MOL GENET,BOSTON,MA 02115
关键词
D O I
10.1128/IAI.64.10.4373-4377.1996
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
A mouse model of Vibrio cholerae infection was successfully developed with germfree mice. Three- to four-week-old germfree mice were orally inoculated with strains of V. cholerae to be tested and then moved to normal housing after inoculation, Stool culture, measurement of serum vibriocidal antibody titers, and determination of immune responses to the cholera toxin B subunit demonstrated that germfree mice are readily colonized by V. cholerae and develop systemic and mucosal immune responses to antigens expressed by these organisms, Immune responses to the B subunit of Shiga toxin 1, which was expressed from a V. cholerae vaccine vector, were less pronounced, This model should be valuable for studying immune responses to V. cholerae infection and immunization, including responses to heterologous antigens expressed by cholera vector strains.
引用
收藏
页码:4373 / 4377
页数:5
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