A set of rhamnosylation-specific antibodies enables detection of novel protein glycosylations in bacteria

被引：8

作者：

Gast, Daniel ^{[1
]}

Koller, Franziska ^{[2
]}

Krafczyk, Ralph ^{[2
]}

Bauer, Lukas ^{[1
]}

Wunder, Swetlana ^{[1
]}

Lassak, Juergen ^{[2
]}

Hoffmann-Roeder, Anja ^{[1
]}

机构：

[1] Ludwig Maximilians Univ Munchen, Dept Chem, Munich, Germany

[2] Ludwig Maximilians Univ Munchen, Dept Biol 1, Microbiol, Planegg Martinsried, Germany

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2020年 / 18卷 / 35期

关键词：

N-GLYCOSYLATION; ARGININE; GENERATION; FLAGELLIN; LINKAGE;

D O I：

10.1039/d0ob01289k

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Despite its potential importance for bacterial virulence, protein rhamnosylation has not yet been sufficiently studied. Specificanti-Ser(Rha),anti-Thr(Rha)andanti-Asn(Rha)antibodies allowed the identification of previously unknown monorhamnosylated proteins in cytosol and membrane fractions of bacterial cell lysates. Mapping of the complete rhamnoproteome in pathogens should facilitate development of targeted therapies against bacterial infections.

引用

页码：6823 / 6828

页数：6

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