A novel method for the analysis of 3-mercaptopyruvate using high-performance liquid chromatography with fluorescence detection

被引：4

作者：

Ogasawara, Yuki ^{[1
]}

Hirokawa, Tomoaki ^{[1
]}

Matsushima, Kaori ^{[1
]}

Koike, Shin ^{[1
]}

Shibuya, Norihiro ^{[2
]}

Tanabe, Shinzo ^{[1
]}

Ishii, Kazuyuki ^{[1
]}

机构：

[1] Meiji Pharmaceut Univ, Dept Hygien Chem, Tokyo 2048588, Japan

[2] Natl Ctr Neurol & Psychiat, Dept Mol Pharmacol, Natl Inst Neurosci, Kodaira, Tokyo 1878502, Japan

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2013年 / 931卷

关键词：

3-Mercaptopyruvate; Cysteine metabolism; Sulfurtransferase; Bound sulfur; Hydrogen sulfide; HYDROGEN-SULFIDE; SULFANE SULFUR; SULFURTRANSFERASE; CYSTEINE; ACID; 3-MERCAPTOLACTATE; BIOCHEMISTRY; URINE; SERUM;

D O I：

10.1016/j.jchromb.2013.05.011

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

3-Mercaptopyruvate (3-MP) is a metabolite of cysteine present in mammalian tissues and is known to be a substrate of 3-mercaptopyruvate sulfurtransferase (3MST, EC.3.4.1.2). The physiological relevance of the 3-MP pathway has not been fully recognized because the metabolic behavior of 3-MP remains unclear. Here, we describe a novel method using high-performance liquid chromatography with fluorescence detection to measure 3-MP formation from cysteine. To demonstrate the practical value of the present method, we applied it to analyze the 3-MP produced in biological samples from mouse tissue. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：56 / 60

页数：5

共 20 条

[1] Novel sources of mammalian C-S lyase activity [J].

Adcock, HJ ;

Gaskin, PJ ;

Shaw, PN ;

TeesdaleSpittle, PH ;

Buckberry, LD .

JOURNAL OF PHARMACY AND PHARMACOLOGY, 1996, 48 (02) :150-153

[2]

AKAGI R, 1982, ACTA MED OKAYAMA, V36, P187

[3] Novel Insights Into Hydrogen Sulfide-Mediated Cytoprotection [J].

Calvert, John W. ;

Coetzee, William A. ;

Lefer, David J. .

ANTIOXIDANTS & REDOX SIGNALING, 2010, 12 (10) :1203-1217

[4]

COOPER AJL, 1982, J BIOL CHEM, V257, P816

[5] 3-MERCAPTOLACTATE CYSTEINE DISULFIDURIA - BIOCHEMICAL-STUDIES ON AFFECTED AND UNAFFECTED MEMBERS OF A FAMILY [J].

HANNESTAD, U ;

MARTENSSON, J ;

SJODAHL, R ;

SORBO, B .

BIOCHEMICAL MEDICINE, 1981, 26 (01) :106-114

[6] DETERMINATION OF 3-MERCAPTOLACTATE, MERCAPTOACETATE AND N-ACETYLCYSTEINE IN URINE BY GAS-CHROMATOGRAPHY [J].

HANNESTAD, U ;

SORBO, B .

CLINICA CHIMICA ACTA, 1979, 95 (02) :189-200

[7] DETERMINATION OF ALPHA-KETO ACIDS IN SERUM AND URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION [J].

HARA, S ;

TAKEMORI, Y ;

YAMAGUCHI, M ;

NAKAMURA, M ;

OHKURA, Y .

JOURNAL OF CHROMATOGRAPHY, 1985, 344 (NOV) :33-39

[8] Hydrogen Sulfide Is a Signaling Molecule and a Cytoprotectant [J].

Kimura, Hideo ;

Shibuya, Norihiro ;

Kimura, Yuka .

ANTIOXIDANTS & REDOX SIGNALING, 2012, 17 (01) :45-57

[9] Hydrogen Sulfide in Biochemistry and Medicine [J].

Lee Predmore, Benjamin ;

Joseph Lefer, David ;

Gojon, Gabriel .

ANTIOXIDANTS & REDOX SIGNALING, 2012, 17 (01) :119-140

[10] Thioredoxin and dihydrolipoic acid are required for 3-mercaptopyruvate sulfurtransferase to produce hydrogen sulfide [J].

Mikami, Yoshinori ;

Shibuya, Norihiro ;

Kimura, Yuka ;

Nagahara, Noriyuki ;

Ogasawara, Yuki ;

Kimura, Hideo .

BIOCHEMICAL JOURNAL, 2011, 439 :479-485

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