Bacille Calmette-Guerin/DNAhsp65 prime-boost is protective against diabetes in non-obese diabetic mice but not in the streptozotocin model of type 1 diabetes

被引:11
作者
daRosa, L. C. [1 ]
Chiuso-Minicucci, F. [1 ]
Zorzella-Pezavento, S. F. G. [1 ]
Franca, T. G. D. [1 ]
Ishikawa, L. L. W. [1 ]
Colavite, P. M. [1 ]
Balbino, B. [1 ]
Tavares, L. C. B. [1 ]
Silva, C. L. [2 ]
Marques, C. [3 ]
Ikoma, M. R. V. [3 ]
Sartori, A. [1 ]
机构
[1] Univ Estadual Paulista, UNESP, Biosci Inst, Dept Microbiol & Immunol, Botucatu, SP, Brazil
[2] Univ Sao Paulo, Dept Biochem & Immunol, BR-14049 Ribeirao Preto, Brazil
[3] Fundacao Dr Amaral Carvalho, Lab Citometria Fluxo, Sao Paulo, Brazil
基金
巴西圣保罗研究基金会;
关键词
BCG; hsp65; immunomodulation; NOD mice; type; 1; diabetes; REGULATORY T-CELLS; HEAT-SHOCK PROTEINS; ANIMAL-MODELS; NOD MICE; INFLAMMATION; MELLITUS; LESSONS; IMMUNOTHERAPIES; PROLIFERATION; AUTOIMMUNITY;
D O I
10.1111/cei.12140
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Type I diabetes is a disease caused by autoimmune destruction of the beta cells in the pancreas that leads to a deficiency in insulin production. The aim of this study was to evaluate the prophylactic potential of a prime-boost strategy involving bacille Calmette-Guerin (BCG) and the pVAXhsp65 vaccine (BCG/DNAhsp65) in diabetes induced by streptozotocin (STZ) in C57BL/6 mice and also in spontaneous type 1 diabetes in non-obese diabetic (NOD) mice. BCG/DNAhsp65 vaccination in NOD mice determined weight gain, protection against hyperglycaemia, decreased islet inflammation, higher levels of cytokine production by the spleen and a reduced number of regulatory T cells in the spleen compared with non-immunized NOD mice. In the STZ model, however, there was no significant difference in the clinical parameters. Although this vaccination strategy did not protect mice in the STZ model, it was very effective in NOD mice. This is the first report demonstrating that a prime-boost strategy could be explored as an immunomodulatory procedure in autoimmune diseases.
引用
收藏
页码:430 / 437
页数:8
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